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Identification of Markers for Dengue Infection in Patients after Visiting Dengue-Endemic Countries
Molecular Genetics, Microbiology and Virology ( IF 0.5 ) Pub Date : 2019-07-01 , DOI: 10.3103/s0891416819030066
V. A. Ternovoi , I. V. Plyasunova , A. O. Sementsova , M. Yu. Kartashov , A. N. Shvalov , E. V. Chausov , L. I. Eremeeva , E. V. Protopopova , E. V. Chub , R. B. Bayandin , O. V. Pyankov , V. B. Loktev , A. P. Agafonov , R. A. Maksyutov

In studied blood serum samples from Russian patients hospitalized with suspected dengue fever, one or more markers of dengue fever have been detected, namely, viral RNA, NS1 antigen, and IgM or IgG antibodies. Moreover, only the combined use of various immunological and genetic methods provided a more effective diagnosis of dengue fever. The detection of IgG to dengue virus in patients with high titers of IgG to tick-borne encephalitis virus (TBEV) and, conversely, the detection of IgG to TBEV virus in patients with high titers of IgG to dengue has been documented. The results confirm the literature data on pronounced antigenic cross-sections between different representatives of flaviviruses, which may complicate the ELISA diagnosis of TBEV and dengue in patients. Genotyping of obtained dengue virus isolates showed that imported cases of dengue fever are caused by subtypes 1–4 of the dengue virus, with the prevalence of infection cases caused by dengue virus 1. Sequencing of these dengue virus isolates, including genome-wide, showed that viral isolates obtained on the territory of Russia are phylogenetically close to modern variants of the dengue virus circulating in the countries of Southeast Asia.

中文翻译:

访问登革热流行国家后患者登革热感染标志物的鉴定

在研究的俄罗斯疑似登革热住院患者的血清样本中,检测到一种或多种登革热标志物,即病毒 RNA、NS1 抗原和 IgM 或 IgG 抗体。此外,只有结合使用各种免疫学和遗传学方法才能提供更有效的登革热诊断。在蜱传脑炎病毒 (TBEV) IgG 滴度高的患者中检测到登革热病毒 IgG,相反,在登革热 IgG 滴度高的患者中检测到 TBEV 病毒 IgG 已有记录。结果证实了有关黄病毒不同代表之间明显抗原横截面的文献数据,这可能会使患者 TBEV 和登革热的 ELISA 诊断复杂化。
更新日期:2019-07-01
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