Abstract

The review is devoted to the analysis of the current achievements of Corynebacterium glutamicum metabolic engineering for the production of lysine. Key genes of lysine biosynthesis in C. glutamicum and ways of creating new genetically modified strains are considered. The role of different plasmids, vector cassettes, and promoter types for the regulation of gene expression in C. glutamicum is described. Information is provided on the use of carbon-containing substrates (hexose, pentose, lactic acid, mannitol) for the production of lysine. Possibilities of using CRISPR technology in genetic engineering of C. glutamicum are considered. Genetic changes in C. glutamicum allowed the use of alternative substrates and contributed to the increase of lysine accumulation in the culture fluid. The data that may be used for the creation of new lysine overproduction strains are summarized.

中文翻译：

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赖氨酸生产谷氨酸棒杆菌菌株的代谢工程

摘要

该评论致力于分析谷氨酸棒杆菌代谢工程在生产赖氨酸方面的最新成就。考虑了谷氨酸棒杆菌赖氨酸生物合成的关键基因以及产生新的基因修饰菌株的方法。描述了不同质粒，载体盒和启动子类型在谷氨酸棒杆菌中调节基因表达的作用。提供了有关使用含碳底物（己糖，戊糖，乳酸，甘露醇）生产赖氨酸的信息。考虑了在谷氨酸棒杆菌的基因工程中使用CRISPR技术的可能性。谷氨酸棒杆菌的遗传变化允许使用其他底物，并有助于增加赖氨酸在培养液中的积累。总结了可用于产生新的赖氨酸过量生产菌株的数据。