Abstract

Using the Comet assay, the peculiarities of the interaction between malignant and normal human peripheral blood lymphocytes under cocultivation and separate cultivation were investigated. A decrease in Tail Moment was observed against an increase in the frequency of cells in the state of apoptosis in the culture of lymphocytes from conditionally healthy volunteers (bystander cells) under the influence of blood cells from patients with CLL (inductor cells). A statistically significant (p < 0.001) reduction both in the frequency of cells with high levels of DNA damages and apoptotic activity was established in the population of inductor cells under the influence of the bystander cells. The results obtained indicate the realization of both direct (effect of cells-inductors on bystander cells) and rescue (effect of bystander cells on cells-inductors) TIBE phenomenon.

中文翻译：

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共培养和单独培养下恶性与正常人外周血淋巴细胞相互作用的评价

摘要

使用彗星试验，研究了在共同培养和单独培养下恶性与正常人外周血淋巴细胞之间相互作用的特殊性。在来自CLL患者的血细胞（诱导细胞）的影响下，观察到尾矩的减少与来自条件健康志愿者（旁观者细胞）的淋巴细胞培养物中处于凋亡状态的细胞频率增加相对。具有统计学意义（p在旁观者细胞的影响下，诱导细胞群中具有高水平DNA损伤的细胞频率和凋亡活性的降低均小于0.001。获得的结果表明实现了直接的（细胞-诱导物对旁观者细胞的影响）和挽救（周围的细胞对旁观者细胞的影响）TIBE现象。