Abstract

This paper presents results of studying the genetic diversity of 126 sunflower inbred lines from the collection fund of the Experimental Farm of Oilseeds of Kazakhstan for protein and SSR markers. The polymorphism of collections in terms of the composition of helianthinin, seed storage protein, was not high. In the zone of fast-moving proteins, three variants of components were found that are characteristic of all types of inbred lines. At the same time, components specific to lines with genes rf and Rf were identified. PCR analysis of DNA of inbred lines by 16 SSR markers showed no variations in the amplification products of locus ORS428 and low polymorphism of locus ORS785 (PIC ≤ 0.2). The following markers in the studied collections showed high polymorphism (PIC > 0.8): ORS316, ORS381, ORS437, ORS456, ORS505, ORS630, ORS761, and ORS925. Based on molecular marking, the registration of inbred lines of the Kazakhstan Collection Fund was carried out. In order to assess the level of hybridity of F1 seeds of commercial sunflower hybrids, SSR markers were selected that can differentiate the genetic material of the original parental lines in the DNA of the hybrid.

中文翻译：

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哈萨克斯坦用于蛋白质和SSR标记的自交系向日葵的遗传多样性

摘要

本文介绍了从哈萨克斯坦油料实验农场收集的126个向日葵自交系中蛋白质和SSR标记的遗传多样性研究结果。就葵花素（种子贮藏蛋白）的组成而言，集合的多态性并不高。在快速移动的蛋白质区域中，发现了三种类型的自交系特征性的组分变体。同时，鉴定了具有基因rf和Rf的品系的特异性成分。的近交系由16个SSR标记DNA的PCR分析表明在基因座的扩增产物没有变化ORS428和基因座的多态性低ORS785（PIC≤0.2）。研究的集合中的以下标记具有较高的多态性（PIC> 0.8）：ORS316，ORS381，ORS437，ORS456，ORS505，ORS630，ORS761和ORS925。基于分子标记，对哈萨克斯坦收藏基金的近交系进行了注册。为了评估商品向日葵杂种的F1种子的杂交水平，选择了可以区分杂种DNA中原始亲本系遗传物质的SSR标记。