Abstract

The natural isolate of xylotrophic basidiomycetes Hericium erinaceus BP 16 was cultivated artificially to produce fruiting bodies from which the polysaccharide-containing fraction (PF) was isolated by extraction with hot water (70°C), followed by precipitation with 96% ethanol. Residues of rhamnose, fructose, xylose, arabinose, mannose, glucose, and galactose in the ratio of 7 : 8 : 1 : 19 : 14 : 26 : 27, respectively, were identified in the composition of the PF carbohydrate chains. The effect of adding PF to aqueous solutions of cryoprotectants during water freezing in human venous blood during its cryopreservation has been demonstrated. It was found that the presence of PF (0.25%) in a glycerin-containing (3.5%) preservative ensures the safety of human nuclear blood cells at −20 and −80°C, which indicated the cryoprotective activity of PF H. erinaceus BP 16. Thus, this property of PF may be used in the development of new substances to freeze biological samples.

中文翻译：

---

蘑菇猴头菇BP 16的多糖级分的低温保护性能。

摘要

木霉菌担子菌天然分离物猴头猴人工培养BP 16以产生子实体，通过用热水（70°C）萃取，然后用96％乙醇沉淀，从中分离出含多糖的馏分（PF）。在PF碳水化合物链的组成中，分别确定了鼠李糖，果糖，木糖，阿拉伯糖，甘露糖，葡萄糖和半乳糖的比例分别为7：8：1：19：14：26：27。已经证明了在冷冻过程中在人静脉血液中水冷冻期间向冷冻保护剂的水溶液中添加PF的效果。已发现，在含甘油（3.5％）的防腐剂中存在PF（0.25％）可确保人核血细胞在-20和-80°C的安全性，这表明PF H. erinaceus的冷冻保护活性 BP 16.因此，PF的这一特性可用于开发新物质以冷冻生物样品。