Kolomyjec et al. (2020). Phylogenetic analysis of Michigan’s freshwater sponges (Porifera, Spongillidae) using extended COI mtDNA sequences. – Zoologica Scripta, 00, 000-000. The phylogenetic relationships of eight species of freshwater sponges sampled throughout the State of Michigan in the North American Great Lakes region were examined as part of a Course-based Undergraduate Research Experience (CURE). An extended version of the standard cytochrome oxidase subunit I (COI) metazoan DNA was used for sequencing and Bayesian phylogenetic inference. The extended gene region (COI-ext) produces a 1,200 bp amplicon instead of the standard 640 bp fragment which compensates for the standard amplicon’s low informatics value in Phylum Porifera. The species examined clustered into strongly supported monophyletic species groups within the family Spongillidae. This study represents the first look at the phylogenetic relationships of freshwater sponges in the Great Lakes Region.

中文翻译：

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使用扩展的COI mtDNA序列进行密歇根州淡水海绵（Porifera，海绵体）的系统发育分析

Kolomyjec等。（2020）。使用扩展的COI mtDNA序列进行密歇根州淡水海绵（Porifera，海绵科）的系统发育分析。– Zoologica Scripta，00，000-000。作为基于课程的本科研究经验（CURE）的一部分，检查了在北美大湖区的整个密歇根州采样的8种淡水海绵的系统发育关系。标准细胞色素氧化酶亚基I（COI）后生动物DNA的扩展版本用于测序和贝叶斯系统发生推断。扩展的基因区域（COI-ext）产生了一个1200 bp的扩增子，而不是标准的640 bp片段，从而弥补了疫霉菌中标准扩增子的低信息学价值。所研究的物种聚集在海绵纲内的强烈支持的单物种群体中。这项研究代表了大湖地区淡水海绵系统发育关系的首次观察。