The biomanufacturing of D-glucaric acid has been attracted increasing interest and the industrial yeast Saccharomyces cerevisiae is regarded as an excellent host for D-glucaric acid production. Here we constructed the biosynthetic pathway of D-glucaric acid in S. cerevisiae INVSc1 whose opi1 was knocked out and obtained two engineered strains, LGA-1 and LGA-C, producing record breaking titers of D-glucaric acid, 9.53 ± 0.46 g/L and 11.21 ± 0.63 g/L D-glucaric acid from 30 g/L glucose and 10.8 g/L myo-inositol in the mode of fed-batch fermentation, respectively. Due to the genetic stability and the outperformance in subsequent applications, however, LGA-1 was a preferable strain. As one of the top chemicals from biomass, there have been no reports on D-glucaric acid production from lignocellulose, which is the most abundant renewable on earth. Therefore, the biorefinery processes of lignocellulose for D-glucaric acid production including separated hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP) were investigated in this work and CBP by an artificial microbial consortium composed of Trichoderma reesei Rut-C30 and S. cerevisiae LGA-1 was found to have relatively high D-glucaric acid titers and yields after 7 d fermentation, 0.54 ± 0.12 g/L D-glucaric acid from 15 g/L Avicel, and 0.45 ± 0.06 g/L D-glucaric acid from 15 g/L steam exploded corn stover (SECS), respectively. In attempts to design the microbial consortium for more efficient CBP the team consisted of the two members, T. reesei Rut-C30 and S. cerevisiae LGA-1, was found to be the best with excellent work distribution and collaboration. This desirable and promising approach for direction production of D-glucaric acid from lignocellulose deserves extensive and in-depth research.

中文翻译：

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人造微生物财团对木质纤维素的合并生物处理以生产葡糖二酸

D-葡糖酸的生物制造已引起越来越多的兴趣，并且工业酵母啤酒酵母被认为是D-葡糖酸生产的优良宿主。在这里，我们构建了酿酒酵母INVSc1中D-葡糖酸的生物合成途径，敲除opi1并获得了两个工程菌株LGA-1和LGA-C，产生了破纪录的D-葡糖酸滴度，为9.53±0.46 g /从30 g / L葡萄糖和10.8 g / L肌球蛋白获得L和11.21±0.63 g / L D-葡萄糖酸分批补料发酵模式下的肌醇。然而，由于遗传稳定性和在后续应用中的优异性能，LGA-1是优选的菌株。作为生物质中最重要的化学物质之一，没有关于木质纤维素生产D-葡萄糖酸的报道，木质纤维素是地球上最丰富的可再生能源。因此，这项工作研究了木质纤维素用于D-葡萄糖酸生产的生物精炼工艺，包括分离的水解和发酵（SHF），同时糖化和发酵（SSF）以及合并的生物加工（CBP），并由木霉菌组成的人工微生物联合体研究了CBP。里氏Rut-C30和酿酒酵母发现LGA-1具有相对较高的D-葡萄糖酸滴度和发酵7天后的产量，15 g / L Avicel的0.54±0.12 g / L D-葡萄糖酸和0.45±0.06 g / L的D-葡萄糖酸15 g / L的蒸汽爆炸玉米秸秆（SECS）。为了设计更高效的CBP微生物联盟，该团队由里氏木霉Rut-C30和酿酒酵母LGA-1这两个成员组成，他们被认为是最佳的，具有出色的工作分配和协作能力。从木质纤维素定向生产D-葡糖酸的这种理想且有希望的方法值得广泛和深入的研究。