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Bioengineered ciprofloxacin loaded chitosan nanoparticles for the treatment of Bovine Mastitis
bioRxiv - Pharmacology and Toxicology Pub Date : 2020-05-10 , DOI: 10.1101/2020.05.09.085563
Preeti Yadav , Preksha Gaur , Awadh B. Yadav , Z. I. Huma , Neelesh Sharma

Mastitis is the most devastating economic disease of dairy cattle. Mastitis in dairy cattle frequently occurs during the dry period or in early lactation. E. coli. and S. aureus is the main causative agent of mastitis. S. aureus has an ability to form encapsulated focuses in the udder and develop subclinical form of mastitis. This property of bacteria hinders the effective cure during lactation period. Antibiotics used for treatments have a short half-life span at the site of action because of frequent milking, therefore unable to maintain desired drug concentration for effective clearance of bacteria. We demonstrated the potential of ciprofloxacin encapsulated nanocarriers which can improve availability of drugs and could provide effective means of the treatment of mastitis. These drug loaded nanoparticles show low toxicity & slow clearance from the site of action. Antimicrobial studied against clinical strain of E.coli. and Staphylococcus aureus zone of inhibition depend on dose 0.5 mg to 2 mg/ml nanoparticles solution from 11.6 to 14.5 mm and 15 to 18mm. These nanoparticles show good antimicrobial activity in broth culture as well against bacteria.

中文翻译:

生物工程化的环丙沙星负载的壳聚糖纳米颗粒用于治疗牛乳腺炎

乳腺炎是奶牛最致命的经济疾病。奶牛的乳腺炎经常发生在干燥时期或哺乳初期。大肠杆菌。和金黄色葡萄球菌是乳腺炎的主要致病因子。金黄色葡萄球菌有能力在乳腺中形成包囊的病灶并发展为乳腺炎的亚临床形式。细菌的这种特性阻碍了泌乳期的有效治愈。由于频繁挤奶,用于治疗的抗生素在作用部位的半衰期很短,因此无法保持有效清除细菌所需的药物浓度。我们证明了环丙沙星包裹的纳米载体的潜力,可以提高药物的利用率,并可以提供治疗乳腺炎的有效手段。这些载有药物的纳米颗粒显示出低毒性和从作用部位清除缓慢。对大肠杆菌的临床菌株进行了抗菌研究金黄色葡萄球菌抑制区域取决于剂量0.5 mg至2 mg / ml纳米颗粒溶液(11.6至14.5 mm和15至18mm)。这些纳米颗粒在肉汤培养物中显示出良好的抗微生物活性,并且对细菌也具有抗微生物活性。
更新日期:2020-05-10
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