Potato spindle tuber viroid and other pospiviroids can cause serious diseases in potato and tomato crops. Consequently, pospiviroids are regulated in several countries. Since seed transmission is considered as a pathway for the introduction and spread of pospiviroids, some countries demand for the testing of seed lots of solanaceous crops for the presence of pospiviroids. A real-time RT-PCR test, named PospiSense, was developed for testing pepper (Capsicum annuum) and tomato (Solanum lycopersicum) seeds for seven pospiviroid species known to occur naturally in these crops. The test consists of two multiplex reactions running in parallel, PospiSense 1 and PospiSense 2, that target Citrus exocortis viroid (CEVd), Columnea latent viroid (CLVd), pepper chat fruit viroid (PCFVd), potato spindle tuber viroid (PSTVd), tomato apical stunt viroid (TASVd), tomato chlorotic dwarf viroid (TCDVd) and tomato planta macho viroid (TPMVd, including the former Mexican papita viroid). Dahlia latent viroid (DLVd) is used as an internal isolation control. Validation of the test showed that for both pepper and tomato seeds the current requirements of a routine screening test are fulfilled, i.e. the ability to detect one infested seed in a sample of c.1000 seeds for each of these seven pospiviroids. Additionally, the Pospisense test performed well in an inter-laboratory comparison, which included two routine seed-testing laboratories, and as such provides a relatively easy alternative to the currently used tests.

中文翻译：

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实时RT-PCR测试方法的开发和验证，用于筛选辣椒和番茄种子中是否存在类胡萝卜素

马铃薯纺锤块茎类病毒和其他类拟寄生虫会在马铃薯和番茄作物上引起严重的疾病。因此，类胡萝卜素在一些国家受到管制。由于种子传播被认为是引入类胡萝卜素的途径，一些国家要求测试茄类作物种子中是否存在类胡萝卜素。开发了一种名为PospiSense的实时RT-PCR测试，用于测试辣椒（Capsicum annuum）和番茄（Solanum lycopersicum））已知在这些农作物中天然存在的7种pospiviroid物种的种子。该测试包含两个并行运行的多重反应，即PospiSense 1和PospiSense 2，分别针对柑橘外皮类病毒（CEVd），圆柱隐性类病毒（CLVd），辣椒聊天水果类病毒（PCFVd），马铃薯纺锤块茎类病毒（PSTVd），番茄顶端特技类病毒（TASVd），番茄绿萎病矮人类病毒（TCDVd）和番茄大地男子气概类病毒（TPMVd，包括前墨西哥的papita类病毒）。大丽花潜伏类病毒（DLVd）用作内部隔离控件。该测试的验证表明，对于胡椒和番茄种子，都满足了常规筛选测试的当前要求，即能够针对这7种类胡萝卜素检测c.1000种子样品中的一种侵染种子。另外，