Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis, but this technique does not work efficiently in other plant species, including Arabidopsis thaliana. As an efficient high-throughput transient expression system is currently lacking in the model plant species A. thaliana, we developed a method that is characterized by high efficiency, reproducibility, and suitability for transient expression of a variety of functional proteins in A. thaliana and 7 other plant species, including Brassica oleracea, Capsella rubella, Thellungiella salsuginea, Thellungiella halophila, Solanum tuberosum, Capsicum annuum, and N. benthamiana. Efficiency of this method was independently verified in three independent research facilities, pointing to the robustness of this technique. Furthermore, in addition to demonstrating the utility of this technique in a range of species, we also present a case study employing this method to assess protein–protein interactions in the sucrose biosynthesis pathway in Arabidopsis.

虽然稳定转化技术的使用已经导致对基因功能的深入了解，但其在高通量研究中的应用仍然很困难。农业渗透已广泛用于本氏烟草等物种，用于快速检测基因表达和蛋白质相互作用分析，但该技术在其他植物物种中无效，包括拟南芥。由于模式植物物种拟南芥目前缺乏高效的高通量瞬时表达系统，我们开发了一种具有高效、可重复和适用于拟南芥中多种功能蛋白瞬时表达的方法以及其他7种植物，包括 甘蓝、荠菜、盐渍藻、嗜盐菌、马铃薯、辣椒、本氏猪笼草。该方法的效率在三个独立的研究机构中得到了独立验证，表明该技术的稳健性。此外，除了证明该技术在一系列物种中的实用性外，我们还介绍了一个案例研究，该研究采用这种方法来评估拟南芥蔗糖生物合成途径中的蛋白质 - 蛋白质相互作用。