Abstract Pichia guilliermondii is a yeast widely found on soil, leaves and fruit surfaces and is known to have a good biocontrol effect on a variety of postharvest diseases of fruits and vegetables. In this study, artificially wounded apple fruits (cv. Fuji) were treated with P. guilliermondii. It was found that P. guilliermondii growth increased rapidly at wound sites of fruit in the early stage of healing and later decreased rapidly. The yeast significantly reduced weight loss of wounded fruit and the disease index of inoculated fruit with Penicillium expansum. On the 5 d of healing, the weight loss and disease index of treated fruits were 25 % and 44 % lower than the control, respectively. P. guilliermondii also promoted the accumulation of O2·− and H2O2, increased the activity of superoxide dismutase, catalase, peroxidase and polyphenol oxidase in fruit wounds. Furthermore, P. guilliermondii activated phenylalnine ammonialyase activity and increased the content of total phenols, flavonoids and lignin in the fruit wounds. It is suggested that postharvest P. guilliermondii treatment could promote the wound-healing of apple fruit by activating the reactive oxygen species and phenylpropane metabolism in the fruit wound.

中文翻译：

---

采后毕赤酵母处理促进苹果果实的伤口愈合

摘要 Pichia guilliermondii 是一种广泛存在于土壤、叶片和果实表面的酵母菌，对多种果蔬采后病害具有良好的生防作用。在这项研究中，人工受伤的苹果果实（cv. Fuji）用 P. guilliermondii 处理。发现P. guilliermondii在愈合早期果实伤口部位生长迅速增加，随后迅速下降。该酵母显着降低了受伤果实的重量损失和接种膨胀青霉的果实的疾病指数。愈合第 5 天，处理后果实的失重和疾病指数分别比对照低 25% 和 44%。P. guilliermondii 还促进了 O2·− 和 H2O2 的积累，增加了超氧化物歧化酶、过氧化氢酶、水果伤口中的过氧化物酶和多酚氧化酶。此外，P. guilliermondii 激活苯丙氨酸解氨酶活性，增加果实伤口中总酚、黄酮类和木质素的含量。提示采后处理可通过激活果实创面的活性氧和苯丙烷代谢促进苹果果实创面愈合。