Brain aging is a complex process, which involves multiple pathways including various components from cellular to molecular. This study aimed to investigate the gene expression changes in zebrafish brains through young-adult to adult, and adult to old age. RNA sequencing was performed on isolated neuronal cells from zebrafish brains. The cells were enriched in progenitor cell markers, which are known to diminish throughout the aging process. We found 176 statistically significant, differentially expressed genes among the groups, and identified a group of genes based on gene ontology descriptions, which were classified as cell adhesion molecules. The relevance of these genes was further tested in another set of zebrafish brains, human healthy, and Alzheimer's disease brain samples, as well as in Allen Brain Atlas data. We observed that the expression change of 2 genes, GJC2 and ALCAM, during the aging process was consistent in all experimental sets. Our findings provide a new set of markers for healthy brain aging and suggest new targets for therapeutic approaches to neurodegenerative diseases.

中文翻译：

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斑马鱼脑 RNA 测序揭示细胞粘附分子对脑衰老至关重要

大脑衰老是一个复杂的过程，涉及多种途径，包括从细胞到分子的各种成分。本研究旨在研究斑马鱼大脑中从成年到成年，从成年到老年的基因表达变化。对来自斑马鱼大脑的分离的神经元细胞进行 RNA 测序。这些细胞富含祖细胞标记物，已知这些标记物在整个衰老过程中会减少。我们在各组之间发现了176个具有统计学意义的差异表达基因，并根据基因本体描述确定了一组基因，这些基因被归类为细胞粘附分子。这些基因的相关性在另一组斑马鱼大脑、人类健康和阿尔茨海默病大脑样本以及艾伦大脑图谱数据中得到了进一步测试。我们观察到2个基因GJC2和ALCAM在衰老过程中的表达变化在所有实验组中都是一致的。我们的研究结果为健康的大脑衰老提供了一套新的标志物，并为神经退行性疾病的治疗方法提出了新的目标。