This study sought to investigate the role of miR-206 in polymyositis/dermatomyositis (PM/DM) development. Transwell and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay were performed to investigate cell migration and proliferation. Real-time polymerase chain reaction (PCR) analysis was used to determine the expression of miR-206, interleukin-17A (IL-17A), IL-17 receptor A (IL-17RA), and regenerating islet-derived protein 3-alpha (REG3A). Significantly, miR-206 mimics decreased macrophage migration and proliferation, while miR-206 inhibitors exhibited the opposite effects. Indeed, elevating IL-17RA levels resulted in increased REG3A expression, which was inhibited by IL-17RA siRNA. Besides, miR-206 mimics decreased IL-17A and REG3A expressions, but miR-206 inhibitors showed opposite effects. Moreover, miR-206 expression in PM/DM patients was significantly reduced compared with the healthy controls, while IL-17A and REG3A expressions substantially increased among PM/DM patients. These findings suggested that downregulation of miR-206 increased the migration and proliferation of macrophages via IL-17A/REG3A signaling pathway, which could promote the inflammatory infiltration in PM/DM.

中文翻译：

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下调miR-206表达通过调节IL-17A/REG3A通路促进巨噬细胞增殖和迁移

本研究旨在调查 miR-206 在多发性肌炎/皮肌炎 (PM/DM) 发展中的作用。进行了 Transwell 和 3-(4,5-二甲基噻唑-2-基)-5-(3-羧基甲氧基苯基)-2-(4-磺基苯基)-2H-四唑鎓内盐 (MTS) 测定以研究细胞迁移和增殖。实时聚合酶链反应 (PCR) 分析用于确定 miR-206、白细胞介素-17A (IL-17A)、IL-17 受体 A (IL-17RA) 和再生胰岛衍生蛋白 3-α 的表达(REG3A)。值得注意的是，miR-206 模拟物降低了巨噬细胞的迁移和增殖，而 miR-206 抑制剂表现出相反的效果。事实上，升高 IL-17RA 水平导致 REG3A 表达增加，这被 IL-17RA siRNA 抑制。此外，miR-206 模拟物降低了 IL-17A 和 REG3A 的表达，但 miR-206 抑制剂表现出相反的效果。此外，与健康对照相比，PM/DM 患者的 miR-206 表达显着降低，而 PM/DM 患者的 IL-17A 和 REG3A 表达显着增加。这些发现表明miR-206的下调通过IL-17A/REG3A信号通路增加巨噬细胞的迁移和增殖，这可能促进PM/DM的炎症浸润。