ABSTRACT

OBJECTIVE: Substance use disorders (SUD) are among the most important public health problems throughout the world. We investigated whether COMT (Val108/158Met), CNR2 (rs2501432 and rs2229579), UCP2 (rs659366), and IL-17 (rs763780) gene variants were associated with SUD and its clinical parameters in a Turkish population.

METHODS: We conducted a case–control study among 136 subjects with SUD and 100 healthy controls. Six variants were analysed by the PCR-RFLP method.

RESULTS: The CNR2 rs2229579 T/T genotype and T allele increased in SUD groups than controls while the C/C genotype and C allele were more prevalent in the control group compared to the SUD group (p = 0.000 and p = 0.001, respectively). The COMT Val108/158Met Val/Val genotype and Val allele were significantly associated with polysubstance abuse (p < 0.05). There was no significant difference between the SUD group and control group regarding genotype and allele frequencies of COMT (Val108/158Met), CNR2 (rs2501432), UCP2 (rs659366) and IL-17 (rs763780) variants.

CONCLUSIONS: This is the first study that discussed the relation of these variants and SUD patients in the Turkish population. The results of the analysis indicated that the CNR2 rs2229579 variant has an effect on susceptibility to SUD, suggesting that this variant might play a role in the physiopathology of SUD. The COMT Val108/158Met variant might be an important factor affecting polysubstance use.

摘要

目的：物质使用障碍（SUD）是全世界最重要的公共卫生问题之一。我们调查了COMT（Val108 / 158Met），CNR2（rs2501432和rs2229579），UCP2（rs659366）和IL-17（rs763780）基因变体是否与SUD及其在土耳其人群中的临床参数有关。

方法：我们在136名SUD患者和100名健康对照者中进行了病例对照研究。通过PCR-RFLP方法分析了六个变体。

结果：与对照组相比，SUD组中CNR2 rs2229579的T / T基因型和T等位基因比对照组增加，而对照组中的C / C基因型和C等位基因比对照组更普遍（分别为p =  0.000和p =  0.001）。 。COMT Val108 / 158Met Val / Val基因型和Val等位基因与多物质滥用显着相关（p  <0.05）。SUD组和对照组在COMT（Val108 / 158Met），CNR2（rs2501432），UCP2（rs659366）和IL-17（rs763780）的基因型和等位基因频率上没有显着差异。

结论：这是讨论土耳其人群中这些变异与SUD患者之间关系的第一项研究。分析结果表明，CNR2 rs2229579变异体对SUD的易感性有影响，表明该变异体可能在SUD的生理病理学中起作用。COMT Val108 / 158Met变体可能是影响多物质使用的重要因素。