ABSTRACT To evaluate the effects of LncRNAZFAS1 on cell proliferation and tumor metastasis in non-small cell lung cancer (NSCLC), we detected the expression level of LncRNAZFAS1 in NSCLC-related tissues and cells. qRT-PCR results revealed that LncRNAZFAS1 in tumor tissues was significantly higher than that in normal lung tissue, especially significantly up-regulated in stage III / IV and in metastatic NSCLC tissues. LncRNAZFAS1 expression was dramatically up-regulated in 4 NSCLC-related cells (A549, SPC-A1, SK-MES-1, and NCI-H1299), with having the highest expression level in A549 cells. Furthermore, we implemented a knockdown of LncRNAZFAS1 in A549 cells, and the results of CCK8 and Transwell assays suggested that knockdown of LncRNAZFAS1 significantly inhibited NSCLC cell proliferation and metastasis. Next, we constructed a tumor xenograft model to evaluate the effect of LncRNAZFAS1 on the NSCLC cell proliferation in vivo. The results indicated that knockdown of LncRNAZFAS1 dramatically inhibited A549 cells proliferation and repressed tumor growth. Additionally, knockdown of LncRNAZFAS1 drastically weakened the expressions of MMP2, MMP9 and Bcl-2 proteins, whereas noticeably strengthened the expression of BAX protein. Our results altogether suggest that knockdown of LncRNAZFAS1 has a negative effect on the proliferation and metastasis of NSCLC cell, which implying LncRNAZFAS1 is a potential unfavorable biomarker in patients with NSCLC.

中文翻译：

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敲低 LncRNAZFAS1 抑制非小细胞肺癌的细胞增殖和转移

摘要 为了评估 LncRNAZFAS1 对非小细胞肺癌 (NSCLC) 细胞增殖和肿瘤转移的影响，我们检测了 LncRNAZFAS1 在 NSCLC 相关组织和细胞中的表达水平。qRT-PCR结果显示肿瘤组织中LncRNAZFAS1显着高于正常肺组织，尤其是在III/IV期和转移性NSCLC组织中显着上调。LncRNAZFAS1 在 4 个 NSCLC 相关细胞（A549、SPC-A1、SK-MES-1 和 NCI-H1299）中表达显着上调，其中 A549 细胞表达水平最高。此外，我们在 A549 细胞中实现了 LncRNAZFAS1 的敲低，CCK8 和 Transwell 测定的结果表明 LncRNAZFAS1 的敲低显着抑制了 NSCLC 细胞的增殖和转移。下一个，我们构建了一个肿瘤异种移植模型来评估 LncRNAZFAS1 对 体内 NSCLC 细胞增殖的影响。结果表明，敲低 LncRNAZFAS1 可显着抑制 A549 细胞增殖并抑制肿瘤生长。此外，敲低 LncRNAZFAS1 显着削弱了 MMP2、MMP9 和 Bcl-2 蛋白的表达，而显着增强了 BAX 蛋白的表达。我们的研究结果表明，敲低 LncRNAZFAS1 对 NSCLC 细胞的增殖和转移具有负面影响，这意味着 LncRNAZFAS1 是 NSCLC 患者中潜在的不利生物标志物。此外，敲低 LncRNAZFAS1 显着削弱了 MMP2、MMP9 和 Bcl-2 蛋白的表达，而显着增强了 BAX 蛋白的表达。我们的研究结果表明，敲低 LncRNAZFAS1 对 NSCLC 细胞的增殖和转移具有负面影响，这意味着 LncRNAZFAS1 是 NSCLC 患者中潜在的不利生物标志物。此外，敲低 LncRNAZFAS1 显着削弱了 MMP2、MMP9 和 Bcl-2 蛋白的表达，而显着增强了 BAX 蛋白的表达。我们的研究结果表明，敲低 LncRNAZFAS1 对 NSCLC 细胞的增殖和转移具有负面影响，这意味着 LncRNAZFAS1 是 NSCLC 患者中潜在的不利生物标志物。