The streaked prochilod Prochilodus lineatus is an important fish species from the Neotropical region. In this study, a protocol of triploidization was established by temperature shock. Fertilized eggs were shocked at 2 min post‐fertilization at 0, 38, 40 and 42°C. At 0°C, the embryos were maintained for 30 min, while the rest for 2 min. Heat shocked embryos and control (during all experiment) were incubated at 27°C. The ploidy status was confirmed by flow cytometry, erythrocyte diameter, conventional cytogenetics (Giemsa staining), chromosome banding and fluorescence in situ hybridization using 5S and 18S rDNA probes. Heat‐shock at 40°C produced 96.7% of triploids and such a procedure did not reduce the fertilization, hatching rate and the percentage of normal embryos. The use of chromosome banding and FISH gave rise to effective procedure to identify triploids. The data obtained here is innovative for the species and bring new information for basic and applied studies.

中文翻译：

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通过流式细胞术、血涂片和核学方法推断的条纹 Prochilodus lineatus 中的三倍体化

有条纹的 prochilod Prochilodus lineatus 是来自新热带地区的重要鱼类。在这项研究中，通过温度冲击建立了三倍体化方案。受精卵在 0、38、40 和 42°C 受精后 2 分钟进行电击。在0°C下，胚胎保持30分钟，其余2分钟。热激胚胎和对照（在所有实验期间）在 27°C 下孵育。倍性状态通过流式细胞术、红细胞直径、常规细胞遗传学（吉姆萨染色）、染色体显带和使用 5S 和 18S rDNA 探针的荧光原位杂交来确认。40°C 的热休克产生了 96.7% 的三倍体，这样的过程不会降低受精率、孵化率和正常胚胎的百分比。染色体显带和 FISH 的使用产生了识别三倍体的有效程序。这里获得的数据对该物种具有创新意义，并为基础和应用研究带来了新信息。