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Characterisation of microsatellite loci in Sardinian pears (Pyrus communis L. and P. spinosa Forssk.)
Scientia Horticulturae ( IF 4.3 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.scienta.2020.109443
Silvia Sau , Chiara Pastore , Guy D’hallewin , Luca Dondini , Gianluigi Bacchetta

Abstract The Sardinian pear germplasm is an important resource of genetic diversity that can be used for increasing data on European pear germplasm, to optimise the efficiency of the association studies within the genome and to identify genomic regions that control the main horticultural traits. A set of 108 Sardinian pear genotypes, composed of 81 Pyrus communis cultivars, 24 wild populations of P. spinosa and three international reference cultivars, was analysed using nine SSR markers to assess the genetic diversity of Sardinian pears, determine their genetic structure and study the cases of synonymies and homonymies. The comparison of SSR profiles indicated four groups of diploid accessions with the same SSR profile. The alignment with the Sardinian dataset, pointed out a clear genotype distinctiveness. For all studied SSR loci, 15 specific rare alleles were identified, with a minimum of two alleles found in the database of analysed accessions for the SSRs EMPc11 and EMPc117. The overall allelic diversity revealed a high polymorphism in the analysed Sardinian germplasm. The structure analysis allowed us to identify four gene pool groups (Sardinian cultivars, Japanese cultivars, late-ripening cultivars and, the most famous, standard cultivars). These results were confirmed by Evanno’s △k statistical analysis which has shown unequivocally that k = 4 (△k = 150) is the most likely stratification level of the cluster. The Q values of P. communis and P. spinosa accessions have confirmed an allelic interchange between wild and cultivated genotypes.

中文翻译:

撒丁梨微卫星位点的表征(Pyrus community L. and P. spinosa Forssk.)

摘要 撒丁梨种质是遗传多样性的重要资源,可用于增加欧洲梨种质的数据,优化基因组内关联研究的效率,并确定控制主要园艺性状的基因组区域。使用 9 个 SSR 标记分析了一组 108 个撒丁梨基因型,由 81 个梨梨栽培品种、24 个野生 P. spinosa 种群和 3 个国际参考栽培品种组成,使用 9 个 SSR 标记分析了撒丁梨的遗传多样性,确定了它们的遗传结构并研究了梨的基因型。同义词和同义词的例子。SSR 谱的比较表明四组二倍体种质具有相同的 SSR 谱。与撒丁岛数据集的一致性指出了明显的基因型独特性。对于所有研究的 SSR 位点,鉴定了 15 个特定的稀有等位基因,在 SSR EMPc11 和 EMPc117 的分析种质数据库中至少发现了两个等位基因。整体等位基因多样性揭示了所分析的撒丁岛种质中的高度多态性。结构分析使我们能够确定四个基因库组(撒丁岛品种、日本品种、晚熟品种和最著名的标准品种)。Evanno 的 △k 统计分析证实了这些结果,该分析明确表明 k = 4 (△k = 150) 是最可能的聚类分层水平。P.communis 和 P. spinosa 种质的 Q 值证实了野生和栽培基因型之间的等位基因互换。整体等位基因多样性揭示了所分析的撒丁岛种质中的高度多态性。结构分析使我们能够确定四个基因库组(撒丁岛品种、日本品种、晚熟品种和最著名的标准品种)。Evanno 的 △k 统计分析证实了这些结果,该分析明确表明 k = 4 (△k = 150) 是最可能的聚类分层水平。P.communis 和 P. spinosa 种质的 Q 值证实了野生和栽培基因型之间的等位基因互换。整体等位基因多样性揭示了所分析的撒丁岛种质中的高度多态性。结构分析使我们能够确定四个基因库组(撒丁岛品种、日本品种、晚熟品种和最著名的标准品种)。Evanno 的 △k 统计分析证实了这些结果,该分析明确表明 k = 4 (△k = 150) 是最可能的聚类分层水平。P.communis 和 P. spinosa 种质的 Q 值证实了野生和栽培基因型之间的等位基因互换。Evanno 的 △k 统计分析证实了这些结果,该分析明确表明 k = 4 (△k = 150) 是最可能的聚类分层水平。P.communis 和 P. spinosa 种质的 Q 值证实了野生和栽培基因型之间的等位基因互换。Evanno 的 △k 统计分析证实了这些结果,该分析明确表明 k = 4 (△k = 150) 是最可能的聚类分层水平。P.communis 和 P. spinosa 种质的 Q 值证实了野生和栽培基因型之间的等位基因互换。
更新日期:2020-08-01
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