Abstract This study investigated changes in endogenous polyamine (PA) content, PA-related amino acid content, and the expression of PA-related genes in different tissues (cap, gill, and stipe) of the edible mushroom (Agaricus bisporus J.E. Lange Imbach) during postharvest storage. The results showed that the putrescine (Put) content increased substantially and continuously in all tissues, and was highest in stipe during storage. Strong positive correlations were observed between Put content and the extent of cap opening. Minor changes in spermidine (Spd) and spermine (Spm) contents were observed during storage, both of which were highest in gills during spore production. PA-related amino acid content and PA-related gene expression were also developmentally and tissue-specifically regulated. The expression levels of AbASS, AbASL, AbARG, and AbOAT increased in gills and reached the highest level when the pileus started cap opening; however, at the same time, in the stipe these expression levels decreased rapidly to the lowest level. The expression levelsof AbODC and AbSAMDC increased in gills with storage duration, which possibly contributed to the highest Spd level. Furthermore, proline and glutamate contents presented an opposite changing trend during storage, indicating that they are likely generated in a coordinated way in different tissues. In addition, Put biosynthetic pathways are likely differently regulated in gill and stipe: Put production in gills mainly happened via ornithine decarboxylase, while Put in stipes may be synthesized via an alternative pathway. These results indicate that PA biosynthesis and its distribution correlate positively with the continuing development of A. bisporus postharvest.

中文翻译：

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双孢蘑菇采后贮藏期间多胺生物合成及不同组织中的分布

摘要 本研究调查了食用菌（Agaricus bisporus JE Lange Imbach）不同组织（菌盖、鳃和菌柄）中内源多胺（PA）含量、PA相关氨基酸含量以及PA相关基因表达的变化。在收获后储存期间。结果表明，腐胺（Put）含量在所有组织中均显着且持续增加，并且在贮藏期间在菌柄中最高。在 Put 含量和开盖程度之间观察到强烈的正相关。在储存期间观察到亚精胺 (Spd) 和精胺 (Spm) 含量的微小变化，在孢子产生期间，这两者在鳃中最高。PA 相关氨基酸含量和 PA 相关基因表达也受到发育和组织特异性调节。AbASS、AbASL、AbARG、菌盖开始开盖时鳃内AbOAT增加并达到最高水平；然而，与此同时，在菌柄中，这些表达水平迅速下降到最低水平。AbODC和AbSAMDC在鳃中的表达水平随着贮藏时间的延长而增加，这可能促成了最高的Spd水平。此外，脯氨酸和谷氨酸含量在储存过程中呈现出相反的变化趋势，表明它们可能在不同组织中以协调方式产生。此外，Put 生物合成途径可能在鳃和菌柄中受到不同的调控：鳃中的 Put 生产主要通过鸟氨酸脱羧酶发生，而菌柄中的 Put 可能通过替代途径合成。