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Evidence of inhibitory effect of Pseudomonas fluorescens CHA0 and aqueous extracts on tomato plants infected with Meloidogyne javanica (Tylenchida: Heteroderidae)
Egyptian Journal of Biological Pest Control ( IF 2.4 ) Pub Date : 2020-02-19 , DOI: 10.1186/s41938-020-00217-0
Ali Moazezikho , Habiballah Charehgani , Mohammad Abdollahi , Rasool Rezaei

Effects of Pseudomonas fluorescens L. (jimsonweed) (Pf) isolate and the two plant extracts, Datura stramonium and Myrtus communis, were investigated on hatching and juvenile (J2s) mortality of Meloidogyne javanica(Tylenchida: Heteroderidae) under laboratory conditions. After determining the values of LC30, LC50, and LC70 of each extract, four leaf stage seedlings of tomato were treated by 20 ml of Pf suspension at a concentration of 108 CFU/ml, using a soil drenching method. After 1 week, the tested plants were inoculated by 4000 eggs and (J2s) of M. javanica and simultaneously were treated by 100 ml of the selected concentrations of D. stramonium (1.1, 1.4, and 1.8%) and M. communis (1.8, 3 and 5.2%), as soil drench. Results showed that a combination of Pf and the leaf extract, D. stramonium at the rate of 1.8% or M. communis at the rate of 5.2%, respectively, reduced the number of eggs per root system and the reproduction factor by 68 and 45%, the number of galls by 64 and 33%, and the number of egg masses by 65 and 43%, than the control. In conclusion, combination of Pf and D. stramonium at the rate of 1.8% or M. communis at the rate of 5.2% can significantly reduce the damage of M. javanica on tomato, under greenhouse conditions.

中文翻译:

荧光假单胞菌CHA0和水提取物对感染爪哇根结线虫的番茄植株的抑制作用的证据(Tylenchida:Heteroderidae)

在实验室条件下,研究了荧光假单胞菌(Jmsonweed)(Pf)分离物和两种植物提取物-曼陀罗(Datura stramonium)和桃金娘(Myrtus communis)的影响,对爪蟾拟南芥(Tylenchida:Heteroderidae)的孵化和少年(J2s)死亡率进行了研究。在确定每种提取物的LC30,LC50和LC70的值后,使用土壤浸湿法,用20 ml Pf悬浮液以108 CFU / ml的浓度处理4个番茄叶期幼苗。1周后,将4000枚卵和(J2s)的爪哇木瓜接种至受试植物,并同时用100 ml选定浓度的地衣棒杆菌(1.1%,1.4%和1.8%)和沙门氏菌(1.8。 ,3和5.2%)作为土壤淋湿。结果表明,Pf和叶提取物D. stramonium的比率为1.8%,Communis。communis的比率为5.2%,与对照相比,分别减少了每个根系的卵数和繁殖率68%和45%,s虫数减少了64%和33%,卵团的数量减少了65%和43%。总之,在温室条件下,以1.8%的Pf和D. stramonium的组合或以5.2%的比例对小菜蛾的组合可以显着降低爪哇分枝杆菌对番茄的伤害。
更新日期:2020-02-19
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