The akirin gene, which is strictly localized in the nucleus, plays a critical role in regulating antimicrobial peptide transcription, and has parallel functions to NF‐κB signaling pathway in both vertebrates and invertebrates. In shrimp, the akirin gene is expressed as innate immunity in response to microbial infection. In the present study, expression of akirin gene in Penaeus monodon with respect to Vibrio harveyi and white spot syndrome virus (WSSV) infections and immunostimulant (β‐glucan) administration were investigated by quantitative polymerase chain reaction. The gene was expressed in various tissue samples of healthy shrimp. Maximum level of expression was immediately after V. harveyi infection, suggesting that it may be an early response gene. Gene expression was remarkably upregulated in the lymphoid organ, gill, and hepatopancreas, whereas downregulation was observed in hemocytes compared with the control. In the case of WSSV‐infected samples, the akirin gene was significantly downregulated in the lymphoid organ but there was no significant difference in expression pattern in hemocytes compared to the control. In gill tissue, maximum expression was observed after 2 hr of infection, the same in hepatopancreas. Experimental challenge of β‐glucan fed shrimp infected with V. harveyi and WSSV resulted in significant upregulation of akirin gene expression in lymphoid and gill tissue.

中文翻译：

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黑虎虾斑节对虾响应免疫刺激和哈维氏弧菌和白斑综合症病毒感染后akirin基因的差异表达

的akirin基因，其在细胞核中严格定位，在调节抗微生物肽的转录中起关键作用，并具有在两个脊椎动物和无脊椎动物平行功能NF-κB信号传导途径。在虾中，akirin基因表达为对微生物感染作出反应的先天免疫力。在本研究中，表达akirin在基因斑节对虾相对于哈氏弧菌和白斑综合征病毒（WSSV）感染和免疫刺激剂（β-D-葡聚糖）给药通过定量聚合酶链反应的影响。该基因在健康虾的各种组织样本中表达。最大表达水平是在哈氏弧菌之后立即表达的感染，提示它可能是早期反应基因。与对照组相比，基因表达在淋巴器官，腮和肝胰腺中显着上调，而在血细胞中观察到下调。对于WSSV感染的样本，淋巴器官中的akirin基因显着下调，但与对照相比，血细胞中的表达模式没有显着差异。在g组织中，感染2小时后观察到最大表达，在肝胰腺中也是如此。用β-葡聚糖喂养的虾受到哈维氏弧菌和WSSV感染的实验挑战导致淋巴和g组织中akirin基因表达显着上调。