The total cultivable microbiota of the ice-stored European sea bass (Dicentrarchus labrax), the most important commercial fish species of the Mediterranean aquaculture, was determined using 16S rRNA gene sequence analysis. High Resolution Melting (HRM) curve profiles and sequencing analysis (V3–V4 region of the 16S rRNA gene) were used respectively for the differentiation and identification of the collected isolates from six time intervals (day 0, 4, 8, 12, 14 and 16) while fish were stored in ice. HRM analysis differentiated the unknown microbiota in ten groups (208 isolates) and in two single isolates based on their HRM curve profiles. The isolates with HRM profiles which were >91% similar within each group were found to belong to the same species using sequencing analysis. Thus, the ten groups consist of representatives of Psychrobacter glacincola, Ps. alimentarius, Ps. cryohalolentis, Ps. maritimus, Ps. fozii, Pseudomonas sp., Paeniglutamicibacter sp., Carnobacterium sp., Leucobacter aridicolis and Bacillus thuringiensis. Based on this approach, Ps. cryohalolentis was found to be the most dominant phylotype at the beginning of fish shelf-life compared to other species. The abundance of this bacterium decreased throughout storage, while Ps. glacincola increased and dominated at the time of the sensory minimum acceptability (day 14) and rejection (day 16). To conclude, HRM could be used for the rapid determination of sea bass microbiota, using the representatives of each group as reference bacterial strains, in order for scientists to solve rapidly stakeholders problems related with microbial quality or safety of fish.

储冰的欧洲鲈鱼（Dicentrarchus labrax）的总可培养菌群）是地中海水产养殖中最重要的商业鱼类，使用16S rRNA基因序列分析确定。高分辨率熔解（HRM）曲线图和测序分析（16S rRNA基因的V3–V4区域）分别用于从六个时间间隔（第0、4、8、12、14和6天）对收集的分离物进行区分和鉴定。 16）将鱼存放在冰中。HRM分析基于其HRM曲线图谱，将未知菌群分为十组（208个分离株）和两个单个分离株。通过测序分析，发现每组中具有> 91％相似性的HRM谱图的分离株属于同一物种。因此，十个小组由Psychrobacter glacincola，Ps的代表组成。法典，诗篇。cryohalolentis，诗篇。maritimus，诗篇。fozii，假单胞菌属，Paeniglutamicibacter属，肉杆菌属，Leucobacter aridicolis和苏云金芽孢杆菌。基于此方法，Ps。与其他物种相比，在鱼类保质期开始时，低温卤虫是最主要的系统型。在整个存储过程中，这种细菌的丰度下降，而Ps则下降。格拉辛科拉在感觉上的最低可接受度（第14天）和拒绝（第16天）时增加并占主导地位。总而言之，HRM可用于以各组代表作为参考细菌菌株快速测定海鲈微生物群，以便科学家迅速解决利益相关者与微生物质量或鱼类安全有关的问题。