Cellular membranes differ in their molecular organisation, shape, and dynamics. Knowing how these properties of membrane architecture relate to the presence and function of specific membrane components is fundamental for understanding membrane-associated cellular processes. Correlative light and electron microscopy (CLEM) is ideally poised to address such problems. Fluorescence microscopy allows identification of cellular membranes through labelled components and can provide temporal information, while electron microscopy allows visualisation of the structure of the same membranes at high resolution. In recent years, various CLEM protocols have been applied to gain insights into cellular membrane architecture. Here, we review conceptually novel approaches by which CLEM has provided insights on membrane reshaping, subcellular localisation of components, host–pathogen interactions, and has answered longstanding mechanistic questions.

细胞膜的分子组织，形状和动力学不同。了解膜结构的这些特性与特定膜组件的存在和功能之间的关系是了解膜相关细胞过程的基础。理想的相关光学和电子显微镜（CLEM）可以解决此类问题。荧光显微镜可以通过标记的成分识别细胞膜，并可以提供时间信息，而电子显微镜可以以高分辨率显示相同膜的结构。近年来，已应用各种CLEM协议来深入了解细胞膜结构。在这里，我们回顾了概念上新颖的方法，CLEM通过这些方法提供了有关膜重塑的见解，