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Diurnal rhythm disruptions induced by chronic unpredictable stress relate to depression-like behaviors in rats.
Pharmacology Biochemistry and Behavior ( IF 3.6 ) Pub Date : 2020-05-11 , DOI: 10.1016/j.pbb.2020.172939
Xin-Ling Wang 1 , De-Quan Wang 2 , Fu-Chao Jiao 3 , Kai-Mo Ding 4 , Yan-Bin Ji 5 , Lin Lu 6 , Kai Yuan 7 , George Fu Gao 8 , Su-Xia Li 2
Affiliation  

The relationship between circadian rhythms and mood disorders has been established, circadian dysregulations are believed to exacerbate the severity of mood disorders and vice versa. Although many studies on diurnal changes of clock genes in animal model of depression have been performed from the RNA level, only a few studies have been carried out from the protein level. In this study, we investigated the diurnal changes induced by chronic unpredictable stress (CUS) using various methods, including free-running wheel test, enzyme-linked immunosorbent assay (ELISA) and Western Blotting (WB). Besides, we examined the depression-like behaviors of rats by sucrose preference test (SPT) and forced swim test (FST). We found that CUS induced significant reductions in the quantity of free-running wheel activity and the amplitude of melatonin secretion rhythm. We also found that CUS induced rhythmic disruptions of clock proteins in hippocampus. Furthermore, we found that the amplitude of PER1 in CA1 was positively related to the severity of depression-like behaviors. These results suggest that stress results in both changes in circadian rhythms and in depression-like behaviors and that it is suggested that these changes are related.



中文翻译:

慢性不可预测的压力引起的昼夜节律紊乱与大鼠的抑郁样行为有关。

已经建立了昼夜节律与情绪障碍之间的关系,据信昼夜失调加重了情绪障碍的严重性,反之亦然。尽管已经从RNA水平对抑郁症动物模型中的时钟基因的昼夜变化进行了许多研究,但是从蛋白质水平仅进行了很少的研究。在这项研究中,我们使用多种方法研究了慢性不可预测压力(CUS)引起的昼夜变化,包括自由运行轮试验,酶联免疫吸附测定(ELISA)和Western Blotting(WB)。此外,我们通过蔗糖偏好测试(SPT)和强迫游泳测试(FST)检查了大鼠的抑郁样行为。我们发现,CUS导致自由轮活动量和褪黑激素分泌节律幅度明显降低。我们还发现,CUS诱导了海马中时钟蛋白的节律性破坏。此外,我们发现,CA1中PER1的振幅与抑郁样行为的严重程度呈正相关。这些结果表明,压力导致昼夜节律的变化和抑郁症行为的改变,并且表明这些变化是相关的。

更新日期:2020-05-11
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