Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) channels by an electrical impedance-based biosensor (CellKey™ system) using CDS. Measures of both potency (EC₅₀) and efficacy (E_max) of these agonists with CellKey™ were almost identical to those made using the traditional Ca²⁺ influx assay in TRPV1- or TRPA1-expressing cells, suggesting that CellKey™ is a simpler and easier means of evaluating TRP activities.

细胞介电谱（CDS）是一项新技术，可通过无标记的基于细胞的测定对多种受体类型进行药理学评估。我们通过使用CDS的基于电阻抗的生物传感器（CellKey™系统）评估了配体门控通道，瞬时受体电势香草酸1（TRPV1）和瞬时受体电势锚蛋白1（TRPA1）通道的活性。用CellKey™对这些激动剂的效价（EC ₅₀）和功效（E _max）的测量与在表达TRPV1或TRPA1的细胞中使用传统的Ca ^2+内流法进行的测量几乎相同，这表明CellKey™更简单评估TRP活动的简便方法。