We report the synthesis and antimicrobial properties of a partially reduced dihydronathphthoquinone analogue of 2-methoxy, 5-acetoxy calamenene, extracted from Subergorgia reticulata. The growth of a pathogenic Vibrio harveyi strain was effectively controlled by the calamenene derivative 1 (Cala1) and its synthetic analog 2 (Cala2). Complete mortality of V. harveyi was observed with 2.5 and 0.5 µg mL-1 concentrations of Cala1 and Cala2, respectively. The metabolic assays demonstrated that Cala1 is a bacteriostatic agent while Cala2 showed bactericidal properties. It was confirmed that translocation of Cala2 into the cytoplasm does not induce any change to the integrity of the bacterial cell wall. The Cala2 induced damage to the genetic material of 70% of cells while genetic material of 91% of cells treated with Cala1 remained intact. The Cala2 is, therefore, proposed as a potential bactericidal compound against the aquaculture pathogen V. harveyi. The fact that the Cala2 exhibited minimal cytotoxicity to Artemia nauplii indicates its potential use as an antimicrobial agent for aquaculture operations.

中文翻译：

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使用cal草烯抑制致病性哈维氏弧菌，该cal草烯来源于印度高粱的网纹茄子及其合成类似物。

我们报告的合成和抗菌性能的部分还原的2-氢甲氧基萘醌类似物2-甲氧基，5-乙酰氧基玉石烯，提取自网贝母（Subergorgia reticulata）。cal草烯衍生物1（Cala1）及其合成类似物2（Cala2）有效地控制了致病性哈维弧菌菌株的生长。用浓度分别为2.5和0.5 µg mL-1的Cala1和Cala2观察到哈维弧菌的完全死亡。代谢测定表明，Cala1是抑菌剂，而Cala2具有杀菌特性。已经证实，Cala2易位到细胞质中不会引起细菌细胞壁完整性的任何改变。Cala2诱导了70％的细胞的遗传物质受损，而用Cala1处理的91％的细胞的遗传物质仍然完好无损。Cala2是，因此，被提议作为针对水产养殖病原体哈维氏菌的潜在杀菌化合物。Cala2对卤虫无节幼体表现出最小的细胞毒性这一事实表明，它潜在地用作水产养殖操作的抗菌剂。