Biotransformation has the advantages of low cost and environmental protection and is a preferred method for production of compounds. At present, most 2,5-dihydroxymethylfuran (DHMF) is synthesized by chemical methods. In this study, 12.008 μg/mL DHMF was produced from 9.045 μg/mL 5-hydroxymethylfurfural (5-HMF) with a yield of 1.33 g/g using the crude enzymes from fungus Ganoderma sessile. To elucidate the toxic potential for both compounds, cytotoxicity tests and acute toxicity were evaluated respectively. 5-HMF induced weak cytotoxicity in HCT-8, A549 and SGC-7901 cells and DHMF exerted no cytotoxicity on HCT-8 while induced inhibition proliferation of A549 and SGC-7901 cells. The acute toxicity study showed no mortality happened in any group even at the single dose of 2000 mg/kg body weight. These results suggest it is feasible to convert 5-HMF to DHMF via crude enzymes from fungus G. sessile under mild condition, and that DHMF displays a potential effect of antitumor in vitro with little acute toxicity.

中文翻译：

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通过灵芝无柄和两种化合物的毒理学评估将5-羟甲基糠醛生物转化为2,5-二羟甲基呋喃。

生物转化具有成本低廉和环境保护的优点，是生产化合物的优选方法。目前，大多数2，5-二羟甲基呋喃（DHMF）是通过化学方法合成的。在这项研究中，使用真菌灵芝无梗的粗酶从9.045μg/ mL的5-羟甲基糠醛（5-HMF）中生产了12.008μg/ mL的DHMF，产量为1.33 g / g。为了阐明两种化合物的潜在毒性，分别评估了细胞毒性试验和急性毒性。5-HMF在HCT-8，A549和SGC-7901细胞中诱导弱细胞毒性，而DHMF对HCT-8没有细胞毒性，而在A549和SGC-7901细胞中则抑制增殖。急性毒性研究表明，即使在单剂量2000 mg / kg体重的情况下，任何一组都没有发生死亡。