BACKGROUND Human herpesviruses (HHV)-6A, HHV-6B and HHV-7 are considered to be involved in the pathogenesis of epilepsy, a common neurological disorder. The objective of this study was to determine the association of roseoloviruses infection with epilepsy. METHODS 53 epilepsy patients and 104 ordinary blood donors were analyzed to determine presence of virus-specific antibodies by enzyme-linked immunosorbent assay (ELISA) and immunofluorescence assay (IFA), genomic sequences, viral load and gene expression by polymerase chain reactions (PCRs) and restriction analysis, HHV-6 protein expression by IFA and level of cytokines by ELISA. RESULTS Roseoloviruses genomic sequences in DNA samples from whole blood were found in 86.8% of patients versus 54.8% of controls and active infection was revealed only in patients with epilepsy (19.6% of roseolovirus-positive patients). Significantly higher viral load and more frequent gene expression was detected in patients compared to the controls. HHV-6-encoded protein expression was demonstrated in 53.3% of patients with previously detected HHV-6 DNA. Changes in level of cytokines were determined in patients with elevated viral load compared to the patients without elevated viral loads and to the controls. CONCLUSIONS Results on frequent active HHV-6 and HHV-7 infection in epilepsy patient' peripheral blood indicate on possible involvement of these viruses in the disease development.

中文翻译：

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成年癫痫患者的持续性玫瑰花叶病毒感染。

背景技术人类疱疹病毒（HHV）-6A，HHV-6B和HHV-7被认为与癫痫的发病机理有关，癫痫是一种常见的神经系统疾病。这项研究的目的是确定玫瑰花叶病毒感染与癫痫的关系。方法对53例癫痫患者和104例普通献血者进行酶联免疫吸附测定（ELISA）和免疫荧光测定（IFA），以检测病毒特异性抗体的存在，通过聚合酶链反应（PCR）测定基因组序列，病毒载量和基因表达限制性酶切分析，IFA检测HHV-6蛋白表达，ELISA检测细胞因子水平。结果在全血DNA样本中发现玫瑰花叶病毒基因组序列的比例为86.8％，而对照为54.8％，仅在癫痫患者中发现了主动感染（19。玫瑰果病毒阳性患者的6％）。与对照组相比，在患者中检测到明显更高的病毒载量和更频繁的基因表达。在53.3％先前检测到HHV-6 DNA的患者中证实了HHV-6编码的蛋白表达。与没有病毒载量升高的患者和对照组相比，确定了病毒载量升高的患者中细胞因子水平的变化。结论癫痫患者外周血中频繁发生活动性HHV-6和HHV-7感染的结果表明，这些病毒可能参与了疾病的发展。与没有病毒载量升高的患者和对照组相比，确定了病毒载量升高的患者中细胞因子水平的变化。结论癫痫患者外周血中频繁发生活动性HHV-6和HHV-7感染的结果表明，这些病毒可能参与了疾病的发展。与没有病毒载量升高的患者和对照组相比，确定了病毒载量升高的患者中细胞因子水平的变化。结论癫痫患者外周血中频繁发生活动性HHV-6和HHV-7感染的结果表明，这些病毒可能参与了疾病的发展。