BACKGROUND Increased awareness of consumers on food safety promotes the development of rapid and sensitive detection techniques for pesticide residues. In this study, a new type of rapid detection card for organophosphorus (OPs) and carbamates (CMs) pesticide residues was developed by electrospinning. The card involved enzyme fiber mat (EFM) and substrate fiber mat (SFM) which were prepared by mixing poly(vinyl alcohol) (PVA) with acetylcholinesterase (AChE) and indolyl acetate (IA), respectively. RESULTS The mean diameter of fibers was 240±53 nm for EFM and 387±84 nm for SFM. Results of ATR-FTIR and XPS confirmed that AChE and IA were successfully encapsulated into the fibers. The minimum concentrations of AChE and IA for effective detection were 1 mg/mL and 3 mg/mL, respectively, and the optimal detection time was 15 min. The limits of detection for this card were 0.5 mg/L for omethoate, 1.5 mg/L for malathion, 0.1 mg/L for carbaryl, and 0.02 mg/L for carbofuran. The detection card exhibited good storage stability and its activity can be maintained when stored at room temperature for at least 4 months. Additionally, the EFM can be reused for three times. CONCLUSION The detection card obtained here was superior to the commercial card in detecting pesticide residues in the real food samples. Hence, this electrospun detection card is potential for simple, rapid and sensitive analysis of pesticide residues. This article is protected by copyright. All rights reserved.

中文翻译：

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一种新型纳米检测卡静电纺丝技术的开发，用于农药残留的快速灵敏分析

背景消费者对食品安全意识的提高促进了农药残留快速灵敏检测技术的发展。本研究采用静电纺丝技术开发了一种新型有机磷（OPs）和氨基甲酸酯（CMs）农药残留快速检测卡。该卡片包括酶纤维垫 (EFM) 和底物纤维垫 (SFM)，它们分别通过将聚乙烯醇 (PVA) 与乙酰胆碱酯酶 (AChE) 和乙酸吲哚酯 (IA) 混合而制备。结果 EFM 的纤维平均直径为 240±53 nm，SFM 的平均直径为 387±84 nm。ATR-FTIR 和 XPS 的结果证实 AChE 和 IA 成功地封装到纤维中。有效检测的 AChE 和 IA 的最低浓度分别为 1 mg/mL 和 3 mg/mL，最佳检测时间为 15 分钟。该卡的检测限为氧化乐果为 0.5 mg/L，马拉硫磷为 1.5 mg/L，甲萘威为 0.1 mg/L，克百威为 0.02 mg/L。该检测卡具有良好的储存稳定性，在室温下至少保存4个月，其活性仍可保持。此外，EFM 可以重复使用 3 次。结论本文获得的检测卡在检测真实食品样品中的农药残留方面优于商用卡。因此，这种静电纺丝检测卡具有简单、快速和灵敏的农药残留分析潜力。本文受版权保护。版权所有。该检测卡具有良好的储存稳定性，在室温下至少保存4个月，其活性仍可保持。此外，EFM 可以重复使用 3 次。结论本文得到的检测卡在检测真实食品样品中的农药残留方面优于商用卡。因此，这种静电纺丝检测卡具有简单、快速和灵敏的农药残留分析潜力。本文受版权保护。版权所有。该检测卡具有良好的储存稳定性，在室温下至少保存4个月，其活性仍可保持。此外，EFM 可以重复使用 3 次。结论本文获得的检测卡在检测真实食品样品中的农药残留方面优于商用卡。因此，这种静电纺丝检测卡具有简单、快速和灵敏的农药残留分析潜力。本文受版权保护。版权所有。农药残留的快速灵敏分析。本文受版权保护。版权所有。农药残留的快速灵敏分析。本文受版权保护。版权所有。