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Enzymatic synthesis of prebiotic carbohydrates from lactose: Kinetics and optimization of transgalactosylation activity of β‐galactosidase from Aspergillus oryzae
Journal of Food Process Engineering ( IF 3 ) Pub Date : 2020-05-08 , DOI: 10.1111/jfpe.13435
Kadir Cinar 1, 2 , Gurbuz Gunes 1 , Haci Ali Gulec 2
Affiliation  

The aim of the study was to analyze galactooligosaccharide (GOS) synthesis catalyzed by β‐galactosidase from Aspergillus oryzae by applying experimental data to optimization and kinetic parameter estimation methods. The apparent kinetic parameters were determined in varying initial lactose concentrations by using six‐step, 11‐parameter GOS synthesis mechanism. The results revealed that GOS yield was mainly dependent on initial lactose concentration; on the other hand, all of the reaction parameters affected the required time to achieve maximum GOS yield (TMGY) significantly. The values of 32 °Brix, 36.8°C, and 8.32 U/g lactose solution were determined as the optimum reaction experimental set which offers 26.73% GOS yield and 369.82 min TMGY. The increased enzyme‐galactose complex dissociation ratio (k3/k−3) with decreasing initial lactose concentration resulted to low GOS productivity although GOS production/GOS dissociation rate ratio (k4/k−4) increased with decreasing initial lactose concentration. This result signified that hydrolysis characteristics dominated the reaction medium at low initial lactose concentration. The role of monosaccharide inhibition became important on GOS synthesis for increasing initial lactose concentration.

中文翻译:

乳糖合成益生元碳水化合物的动力学:米曲霉β-半乳糖苷酶的动力学和半乳糖苷酶活性的优化

该研究的目的是通过将实验数据应用于优化和动力学参数估算方法,来分析米曲霉中β-半乳糖苷酶催化的半乳糖低聚糖(GOS)合成。通过使用六步,11参数的GOS合成机理,在变化的初始乳糖浓度下确定了表观动力学参数。结果表明,GOS产量主要取决于初始乳糖浓度。另一方面,所有反应参数都会影响达到最大GOS产量(TMGY)所需的时间。确定了32°Brix,36.8°C和8.32 U / g乳糖溶液的值作为最佳反应实验装置,可提供26.73%的GOS收率和369.82分钟的TMGY。酶半乳糖复合物解离率增加(尽管初始乳糖浓度降低,但GOS产量/ GOS解离速率比(k 4 / k -4)升高,初始乳糖浓度降低的k 3 / k -3导致了低GOS生产率。该结果表明,在低初始乳糖浓度下,水解特性主导了反应介质。单糖抑制的作用对于增加初始乳糖浓度的GOS合成很重要。
更新日期:2020-05-08
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