In the current study, electrospun poly(ε‐caprolactone)‐gelatin (PCL‐Gel) fibrous scaffolds containing magnesium oxide (MgO) particles and preseeded with human endometrial stem cells (hEnSCs) were developed to use as wound care material in skin tissue engineering applications. Electrospun fibers were fabricated using PCL‐Gel (1:1 [wt/wt]) with different concentrations of MgO particles (1, 2, and 4 wt%). The fibrous scaffolds were evaluated regarding their microstructure, mechanical properties, surface wettability, and in vitro and in vivo performances. The full‐thickness excisional wound model was used to evaluate the in vivo wound healing ability of the fabricated scaffolds. Our findings confirmed that the wounds covered with PCL‐Gel fibrous scaffolds containing 2 wt% MgO and preseeded with hEnSCs have nearly 79% wound closure ability while sterile gauze showed 11% of wound size reduction. Our results can be employed for biomaterials aimed at the healing of full‐thickness skin wounds.

中文翻译：

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预接种子宫内膜干细胞的高多孔电纺聚（ε-己内酯）/明胶/MgO 支架促进全层皮肤伤口组织再生：一项体内研究。

在目前的研究中，电纺聚（ε-己内酯）-明胶（PCL-Gel）纤维支架含有氧化镁（MgO）颗粒并预先接种了人类子宫内膜干细胞（hEnSCs），被开发用作皮肤组织工程中的伤口护理材料应用程序。使用 PCL-Gel (1:1 [wt/wt]) 和不同浓度的 MgO 颗粒 (1、2 和 4 wt%) 制造电纺纤维。对纤维支架的微观结构、机械性能、表面润湿性以及体外和体内性能进行了评估。全层切除伤口模型用于评估制造的支架的体内伤口愈合能力。我们的研究结果证实，用含有 2 wt% MgO 的 PCL-Gel 纤维支架覆盖并预先接种 hEnSCs 的伤口具有近 79% 的伤口闭合能力，而无菌纱布显示伤口尺寸减少 11%。我们的结果可用于旨在治愈全层皮肤伤口的生物材料。