Curdlan or laminarin, a β-1,3-glucan was hydrolysed by β-1,3-endoglucanase (CtLam81A) from Clostridium thermocellum to produce laminari-oligosaccharides. TLC analysis of hydrolysed curdlan showed the presence of laminari-oligosaccharides of the degree of polymerization, DP2-DP7. This mixture of laminari-oligosaccharides displayed prebiotic properties. Laminari-oligosaccharides showed an increase in the growth of probiotic bacteria such as Lactobacillus plantarum DM5 and Lactobacillus acidophilus, while they did not promote the growth of non-probiotic bacteria (Escherichia coli and Enterobacter aerogenes). Laminari-oligosaccharides showed higher prebiotic activity score of 0.92 ± 0.01 and 0.64 ± 0.08 for L. plantarum DM5 and L. acidophilus NRRL B-4496, respectively, similar to those shown by inulin. Laminari-oligosaccharides showed higher resistance or low digestibility against α-amylase, artificial gastric juice and intestinal fluid than inulin indicating their bioavailability to the probiotic bacteria present in the gastrointestinal tract of human. The probiotic bacteria consumed laminaribiose and laminariotriose more readily than higher laminari-oligosaccharides as carbon source for their growth. The in vitro cytotoxicity assay of laminari-oligosaccharides (1 mg/ml) on human embryonic kidney (HEK 293) cells showed that the cell viability was not affected even after 72 h indicating their biocompatible nature. All the results amply indicated that laminari-oligosaccharides can serve as potential prebiotic additives for functional food products.

中文翻译：

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热纤梭菌中β-1,3-内葡聚糖酶从柯德兰产生的层状低聚糖的体外益生元潜力，消化率和生物相容性。

Curdlan或laminarin中的β-1,3-葡聚糖被热纤梭菌中的β-1,3-内切葡聚糖酶（CtLam81A）水解，生成片状低聚糖。水解的凝胶多糖的TLC分析显示存在聚合度为DP2-DP7的层状低聚糖。海带-低聚糖的混合物显示出益生元的特性。Laminari-低聚糖显示益生菌（例如植物乳杆菌DM5和嗜酸乳杆菌）的生长增加，而它们却没有促进非益生菌（大肠杆菌和产气肠杆菌）的生长。层状低聚糖显示植物乳杆菌DM5和嗜酸乳杆菌NRRL B-4496的较高的益生元活性评分分别为0.92±0.01和0.64±0.08，与菊粉显示的相似。层板低聚糖比菊粉对α-淀粉酶，人造胃液和肠液表现出更高的抗性或低消化率，这表明它们对人胃肠道中益生菌的生物利用度高。益生菌比高含量的海带寡糖更容易消耗海藻糖和海藻糖作为生长的碳源。层板低聚糖（1 mg / ml）对人胚肾（HEK 293）细胞的体外细胞毒性试验表明，即使72小时后，细胞活力也没有受到影响，表明它们具有生物相容性。所有结果充分表明，海带低聚糖可以作为功能性食品的潜在益生元添加剂。人工胃液和肠液比菊粉表明其对人类胃肠道中存在的益生菌的生物利用度。益生菌比高含量的海带寡糖更容易消耗海藻糖和海藻糖作为生长的碳源。层板低聚糖（1 mg / ml）对人胚肾（HEK 293）细胞的体外细胞毒性试验表明，即使72小时后细胞活力也没有受到影响，表明它们具有生物相容性。所有结果充分表明，海带低聚糖可以作为功能性食品的潜在益生元添加剂。人工胃液和肠液比菊粉表明其对人类胃肠道中存在的益生菌的生物利用度。益生菌比高含量的海带寡糖更容易消耗海藻糖和海藻糖作为生长的碳源。层板低聚糖（1 mg / ml）对人胚肾（HEK 293）细胞的体外细胞毒性试验表明，即使72小时后细胞活力也没有受到影响，表明它们具有生物相容性。所有结果充分表明，海带低聚糖可以作为功能性食品的潜在益生元添加剂。益生菌比高层的低聚寡糖更容易消耗层状的二糖和层状的三糖作为生长的碳源。层板低聚糖（1 mg / ml）对人胚肾（HEK 293）细胞的体外细胞毒性试验表明，即使72小时后细胞活力也没有受到影响，表明它们具有生物相容性。所有结果充分表明，海带低聚糖可以作为功能性食品的潜在益生元添加剂。益生菌比高层的低聚寡糖更容易消耗层状的二糖和层状的三糖作为生长的碳源。层板低聚糖（1 mg / ml）对人胚肾（HEK 293）细胞的体外细胞毒性试验表明，即使72小时后细胞活力也没有受到影响，表明它们具有生物相容性。所有结果充分表明，海带低聚糖可以作为功能性食品的潜在益生元添加剂。