Organosulfur compounds (OSCs) of garlic have various health benefits including anti-hypertensive effect. It has been described that volatile OSCs uptake occurs into a moderate extent. Among the bioavailable OSCs present in garlic, 2-vinyl-4H-1,3-dithiin (2VD) is a main component in garlic macerated oil and stir-fried garlic but has been poorly investigated compared with others OSCs, such allyl mercaptan (AM) and S-allyl cysteine (SAC). The aim of this study was to evaluate the effects of 2VD on vascular smooth muscle cells (VSMCs) isolated from spontaneous hypertensive rats (SHR) and compare them with those produced by AM and SAC. Cell viability and proliferation were measured using tetrazolium dye MTT assay and flow cytometry. Cell migration was evaluated by scrape-wound migration assay. OSCs anti-oxidative capacity was determined by measuring reactive oxygen species (ROS) generation, and total antioxidant status (TAS). Non-toxic plasmatic concentrations (10 μg L−1) of 2VD and AM inhibited VSMCs proliferation stimulated with 5% fetal bovine serum and impaired cell migration. In further flow cytometry analysis 2VD treatment resulted in a partial cell cycle arrest at G2 phase. The OSCs tested were able to prevent ROS increase after angiotensin II stimulation and surprisingly 2VD induced higher total antioxidant status compared with AM and SAC. Our results showed that 2VD produces equivalent or superior beneficial effects on VSMCs to those reported for other bioavailable compounds of garlic. This preliminary evidence suggests that 2VD intake could also exert important protective effects against arterial remodeling in hypertension.

中文翻译：

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2-Vinyl-4H-1,3-Dithiin，一种来自大蒜的生物可利用化合物，通过减少氧化应激来抑制血管平滑肌细胞的增殖和迁移

大蒜的有机硫化合物 (OSC) 具有多种健康益处，包括抗高血压作用。据描述，挥发性 OSCs 吸收发生在中等程度。在大蒜中存在的生物可利用的 OSCs 中，2-vinyl-4H-1,3-dithiin (2VD) 是大蒜浸渍油和炒大蒜的主要成分，但与其他 OSCs 相比，如烯丙基硫醇 (AM ) 和 S-烯丙基半胱氨酸 (SAC)。本研究的目的是评估 2VD 对自发性高血压大鼠 (SHR) 分离的血管平滑肌细胞 (VSMC) 的影响，并将其与 AM 和 SAC 产生的细胞进行比较。使用四唑鎓染料 MTT 测定和流式细胞术测量细胞活力和增殖。通过刮伤迁移测定评估细胞迁移。OSCs 的抗氧化能力是通过测量活性氧 (ROS) 的产生和总抗氧化状态 (TAS) 来确定的。2VD 和 AM 的无毒血浆浓度 (10 μg L-1) 抑制了用 5% 胎牛血清刺激的 VSMC 增殖和细胞迁移受损。在进一步的流式细胞术分析中，2VD 处理导致部分细胞周期停滞在 G2 期。所测试的 OSC 能够防止血管紧张素 II 刺激后 ROS 增加，并且与 AM 和 SAC 相比，令人惊讶的是 2VD 诱导了更高的总抗氧化状态。我们的结果表明，2VD 对 VSMC 产生的有益作用与报道的其他大蒜生物可利用化合物的有益作用相同或更好。这一初步证据表明，摄入 2VD 也可以对高血压的动脉重塑发挥重要的保护作用。