Syndecan-1, is a transmembrane heparan/chondroitin sulfate proteoglycan necessary for cell-cell and cell-matrix interactions. Its decreased level on the cell surface correlates with poor prognosis in several tumor types. Aberrant stromal localization of syndecan-1 is also considered an unfavorable prognostic factor in various human malignancies. In the presented work the question was addressed if changes in syndecan-1 expression are related to the prognosis of cervical cancer. Immunohistochemistry for syndecan-1 extracellular domain was performed on surgical specimens of primary cervical cancer. To follow the communication between tumor cells and stromal fibroblasts, their mono-and co-cultures were studied, detecting the expression of syndecan-1, smooth muscle actin, vimentin, and desmin. Immunohistochemistry of tumorous specimens revealed that while cell surface syndecan-1 expression was reduced on cancer cells, it appeared on the surface of tumor-associated fibroblasts. Until year 7, the cohort with high cell surface syndecan-1 expression had significantly longer survival. No difference in the same time-period could be detected when stromal syndecan-1 expression was analyzed. In vitro analysis revealed, that tumor cells can induce syndecan-1 expression on fibroblast, and fibroblasts showed that fibroblast-like cells are built by two cell types: (a) syndecan-1 positive, cytokeratin negative real fibroblasts, and (b) syndecan-1 and cytokeratin positive epithelial-mesenchymal transformed tumor cells. Syndecan-1 on the surface of cancer cells appears to be a positive prognostic marker. Although syndecan-1 positive fibroblasts promote tumor cell proliferation in vitro, we failed to detect their cancer promoting effect in vivo.

中文翻译：

---

Syndecan-1在宫颈癌中的异常表达。

Syndecan-1是一种跨膜肝素/硫酸软骨素蛋白聚糖，是细胞-细胞和细胞-基质相互作用所必需的。它在细胞表面的水平降低与几种肿瘤类型的预后不良有关。Syndecan-1的异常基质定位在各种人类恶性肿瘤中也被认为是不利的预后因素。在提出的工作中，要解决的问题是syndecan-1表达的变化是否与宫颈癌的预后有关。在原发性宫颈癌的手术标本上进行了syndecan-1细胞外结构域的免疫组织化学分析。为了追踪肿瘤细胞与基质成纤维细胞之间的交流，研究了它们的单培养和共培养，检测了syndecan-1，平滑肌肌动蛋白，波形蛋白和结蛋白的表达。肿瘤标本的免疫组织化学显示，虽然癌细胞表面syndecan-1的表达减少，但它在肿瘤相关的成纤维细胞表面上表达。直到第7年，具有高细胞表面syndecan-1表达的队列的生存期明显延长。分析基质syndecan-1表达时，在同一时间段内未检测到差异。体外分析显示，肿瘤细胞可以诱导成纤维细胞上的syndecan-1表达，成纤维细胞显示成纤维细胞样细胞由两种细胞类型构建：（a）syndecan-1阳性，细胞角蛋白阴性的真实成纤维细胞，以及（b）syndecan -1和角蛋白阳性的上皮间质转化的肿瘤细胞。癌细胞表面上的Syndecan-1似乎是阳性的预后标志物。