Abstract

This study dealt with the fabrication of an impedimetric biosensor based on nanomaterial modified with pencil graphite electrode for the detection of tetracycline (TET) in milk samples. For response of the impedimetric aptasensor to be improved, the influence of different parameters (immobilization time of reduced grapheme oxide, time of aptamer, and TET binding, and concentration of aptamer) was optimized. In optimum conditions, the aptasensor provided a concentration range within 1 × 10^–16 − 1 × 10^–6 M and with a limit of detection of 3 × 10^–17 M TET. The proposed impedimetric aptasensor was then used in milk samples analysis, and the acceptable recovery was achieved ranging from 92.8 to 102.1%. According to this study, the combination of an aptamer and electrochemical impedance spectroscopy is a promising method for detection of TET in milk samples with high reproducibility and stability.

Graphic abstract

中文翻译：

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用于阻抗法检测牛奶中四环素的电化学生物传感器的研制

摘要

本研究涉及基于石墨电极修饰的纳米材料的阻抗式生物传感器的制造，用于检测牛奶样品中的四环素 (TET)。为了提高阻抗适体传感器的响应，优化了不同参数（还原氧化石墨烯的固定时间、适体的时间、TET结合时间和适体浓度）的影响。在最佳条件下，适体传感器提供的浓度范围在 1 × 10 ^–16 − 1 × 10 ^–6  M 内，检测限为 3 × 10 ^–17 M TET。然后将所提出的阻抗适体传感器用于牛奶样品分析，并达到了可接受的回收率，范围从 92.8% 到 102.1%。根据这项研究，适体和电化学阻抗谱的结合是检测牛奶样品中 TET 的一种有前途的方法，具有高重现性和稳定性。

图形摘要