The purpose of this study was to evaluate the possible synergistic effect of gold nano-therapy (Au-NPs) on Y79 cells treated with ultrasonic hyperthermia. The cells in the presence and absence of gold nanoparticles were subjected to ultrasonic irradiation (3 MHz, 1 W/cm 2 , 40% pulse mode). Gold nanoparticles with an average diameter of 60 nm were synthesized. The cell viability of the Y79 cells was evaluated 48 h after performing 0.5–11 min hyperthermia with and without Au-NPs using the MTT assay. There was no cytotoxicity with 1.7 μg/mL Au-NP concentration. Moreover, the results revealed a significant difference in the cell viability between the control group and the groups with a hyperthermia period of more than 4 min. The results indicated that with increasing the concentration of Au-NPs (1.75, 3.5, 7, 14, 28 and 56 μg/mL), the cytotoxicity increased. The Y79 cell viability of 50% was caused for 9 min hyperthermia time alone and 4.5 min hyperthermia time in the presence of gold nanoparticles. The cell viability of the Y79 cells for a 0.5 to 11 min hyperthermia time, compared with the control group, was 93 to 27% and 95 to 32% with and without Au-NPs, respectively. It is concluded that the cells are more sensitive to hyperthermia induced by ultrasound irradiation in the presence of Au-NPs.

中文翻译：

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金纳米粒子超声热疗对视网膜母细胞瘤Y79细胞的影响

本研究的目的是评估金纳米疗法 (Au-NPs) 对用超声热疗处理的 Y79 细胞可能产生的协同作用。在存在和不存在金纳米粒子的情况下对细胞进行超声照射（3 MHz，1 W/cm 2 ，40% 脉冲模式）。合成了平均直径为 60 nm 的金纳米粒子。使用 MTT 测定在使用和不使用 Au-NP 进行 0.5-11 分钟热疗后 48 小时评估 Y79 细胞的细胞活力。1.7 μg/mL Au-NP 浓度没有细胞毒性。此外，结果显示对照组和热疗时间超过 4 分钟的组之间的细胞活力存在显着差异。结果表明，随着 Au-NPs 浓度的增加（1.75、3.5、7、14、28 和 56 μg/mL），细胞毒性增加。50% 的 Y79 细胞活力是由单独的 9 分钟热疗时间和在金纳米粒子存在下 4.5 分钟的热疗时间引起的。与对照组相比，在 0.5 到 11 分钟的热疗时间内，Y79 细胞的细胞活力在有和没有 Au-NP 的情况下分别为 93 到 27% 和 95 到 32%。得出的结论是，在 Au-NPs 存在的情况下，细胞对超声照射引起的高热更敏感。