The use of lignocellulosic wastes reduces dependence on fossil fuel resources, contributes to sustainable waste management, and reinforces the circular economy model of continual use of resources. Typically, the second generation of bioethanol production involves several steps to transform lignocellulosic material into bioethanol. The more complicated step of the overall process is to define a tailor-made to each lignocellulosic material available with a wide variety of complex structures of the lignin, hemicellulose, and cellulose. However, the thermochemicals are the most frequently reported pretreatments, and they have the bottleneck of producing an additional waste stream with a high charge of pollution owing to chemical products implicated. This problem harms on the zero waste policy that we must include in our technological process to be considered sustainable and ecological processes. Consolidated bioprocessing (CBP) is a viable alternative to produce bioethanol from lignocellulosic materials, using a single microorganism, in one-step, and no chemical products are implied. Three agro-industrial wastes with lignocellulosic characteristics were evaluated as a substrate for bioethanol production with a Mexican native white-rot fungus Trametes hirsuta CS5 in a one-step process. Qualitative and quantitative analyses of lignocellulolytic enzymes produced by native fungus were carried out. Instead, Trametes hirsuta is a lignin degrader white-rot fungus; it was capable to produce until 500 U/L of cellulase titers and a maximum xylanase activity of 45 U/mL when it was cultivated in orange peel substrate. Substantial ethanol yields were achieved using lignocellulosic materials like brewer’s spent grain (BSG), orange peel (OP), and wheat bran (WB) as a carbon source in fermentation with no chemicals, which represents a zero waste environment-friendly ethanol production system. Ethanol yield on wheat bran was the highest of all evaluated substrates, reaching value of 34.9% at 7 days, being T. hirsuta able to degrade other hexoses and pentoses present in the structural polymers of cellulose and hemicellulose.

木质纤维素废料的使用减少了对化石燃料资源的依赖，有助于可持续的废料管理，并加强了持续使用资源的循环经济模式。通常，第二代生物乙醇生产涉及将木质纤维素材料转化为生物乙醇的几个步骤。整个过程中更复杂的步骤是为每种木质纤维素材料定义一种量身定制的材料，其中包含木质素，半纤维素和纤维素的各种复杂结构。然而，热化学试剂是最常报道的预处理方法，并且由于涉及化学产品，它们具有产生额外的废物流和大量污染的瓶颈。这个问题损害了零废物政策，我们必须将其纳入我们的技术过程中，才能将其视为可持续的生态过程。整合生物处理（CBP）是一种可行的替代方法，可使用单一微生物一步一步从木质纤维素材料生产生物乙醇，并且不暗示任何化学产品。评价了三种具有木质纤维素特性的农业工业废料，作为墨西哥本地白腐真菌生产生物乙醇的底物一步部署Trametes hirsuta CS5。对天然真菌产生的木质纤维素分解酶进行了定性和定量分析。相反，Trametes hirsuta是一种木质素降解白腐真菌。在橙皮基质中培养时，它能够产生直至500 U / L的纤维素酶滴度，最大木聚糖酶活性为45 U / mL。使用木质纤维素材料（如啤酒的废谷物（BSG），桔皮（OP）和麦麸（WB））作为无化学物质发酵的碳源，可以实现相当大的乙醇产量，这代表了零废物，环境友好的乙醇生产系统。麦麸上的乙醇产量是所有评估的底物中最高的，在7天时达到了T. hirsuta值34.9％ 能够降解纤维素和半纤维素的结构聚合物中存在的其他己糖和戊糖。