The primary motivation behind this study was to explore the energy potential of the residual defatted microalgae biomass by anaerobic co-digestion with rice straw (RS). Two defatted microalgae species Chlorella CG12 and Desmodesmus GS12 were co-digested with RS at C/N ratio of 30. A considerable cumulative biomethane yield of 382 mL/g-VS obtained in the co-digestion of CG12 + RS followed by GS12 + RS (311 mL/g-VS) shows 49.87% and 22.26% increment, respectively, as compared to the control. The sludge on the 40th day of the anaerobic digestion was reutilized to cultivate the fresh batch of microalgae. The microalgal growth was studied at a light intensity of 3000 Lux, 22 ± 2 °C and pH of 7–7.5. The maximum lipid content of 19% on a dry cell weight basis (dcw) was seen at 20% sludge concentration in GS12. Total microalgal biomass was found to be increased by 22% compared to the control, i.e., in BG-11 media. This combined use of microalgae with RS helped in enhanced energy recovery. It also provides a sustainable approach for the development of a microalgae-based biorefinery for the production of biodiesel and biogas as an energy fuel.

On the integrated biorefinery set-up approach for use of defatted microalgal in sustainable biofuel production.

这项研究的主要动机是通过与稻草（RS）厌氧共消化来探索残留的脱脂微藻生物质的能量潜力。两种脱脂微藻物种小球藻CG12和DesmodesmusGS12与RS以30的C / N比共同消化。通过CG12 + RS共消化，然后GS12 + RS（311 mL / g-VS）共消化，可观的累积生物甲烷产量为382 mL / g-VS。与对照相比，分别显示出49.87％和22.26％的增量。厌氧消化第40天的污泥被重新利用以培养新鲜的微藻批次。在3000 Lux，22±2°C和pH 7-7.5的光强度下研究了微藻的生长。在GS12中，当污泥浓度为20％时，以干细胞重量（dcw）计，最大脂质含量为19％。发现相比于对照，即在BG-11培养基中，总微藻生物量增加了22％。微藻与RS的结合使用有助于提高能量回收率。

关于将脱脂微藻用于可持续生物燃料生产的综合生物精炼厂设置方法。