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Short- and Long-Term Transcriptomic Responses of Escherichia coli to Biocides: a Systems Analysis.
Applied and Environmental Microbiology ( IF 4.4 ) Pub Date : 2020-07-02 , DOI: 10.1128/aem.00708-20 Beatriz Merchel Piovesan Pereira 1, 2 , Xiaokang Wang 2, 3 , Ilias Tagkopoulos 4, 5
Applied and Environmental Microbiology ( IF 4.4 ) Pub Date : 2020-07-02 , DOI: 10.1128/aem.00708-20 Beatriz Merchel Piovesan Pereira 1, 2 , Xiaokang Wang 2, 3 , Ilias Tagkopoulos 4, 5
Affiliation
The mechanisms of the bacterial response to biocides are poorly understood, despite their broad application. To identify the genetic basis and pathways implicated in the biocide stress response, we exposed Escherichia coli populations to 10 ubiquitous biocides. By comparing the transcriptional responses between a short-term exposure (30 min) and a long-term exposure (8 to 12 h) to biocide stress, we established the common gene and pathway clusters that are implicated in general and biocide-specific stress responses. Our analysis revealed a temporal choreography, starting from the upregulation of chaperones to the subsequent repression of motility and chemotaxis pathways and the induction of an anaerobic pool of enzymes and biofilm regulators. A systematic analysis of the transcriptional data identified a zur-regulated gene cluster to be highly active in the stress response against sodium hypochlorite and peracetic acid, presenting a link between the biocide stress response and zinc homeostasis. Susceptibility assays with knockout mutants further validated our findings and provide clear targets for downstream investigation of the implicated mechanisms of action.
中文翻译:
大肠杆菌对杀菌剂的短期和长期转录组反应:系统分析。
尽管杀菌剂应用广泛,但细菌对其反应的机制却知之甚少。为了确定杀菌剂应激反应中涉及的遗传基础和途径,我们将大肠杆菌群体暴露于 10 种普遍存在的杀菌剂中。通过比较短期暴露(30 分钟)和长期暴露(8 至 12 小时)对杀菌剂应激的转录反应,我们建立了与一般和杀菌剂特异性应激反应有关的常见基因和通路簇。我们的分析揭示了一种时间编排,从伴侣蛋白的上调开始,到随后对运动和趋化途径的抑制,以及酶和生物膜调节剂厌氧池的诱导。对转录数据的系统分析发现,zur调节的基因簇在针对次氯酸钠和过乙酸的应激反应中高度活跃,表明杀菌剂应激反应与锌稳态之间的联系。敲除突变体的敏感性测定进一步验证了我们的发现,并为下游研究所涉及的作用机制提供了明确的目标。
更新日期:2020-07-02
中文翻译:
大肠杆菌对杀菌剂的短期和长期转录组反应:系统分析。
尽管杀菌剂应用广泛,但细菌对其反应的机制却知之甚少。为了确定杀菌剂应激反应中涉及的遗传基础和途径,我们将大肠杆菌群体暴露于 10 种普遍存在的杀菌剂中。通过比较短期暴露(30 分钟)和长期暴露(8 至 12 小时)对杀菌剂应激的转录反应,我们建立了与一般和杀菌剂特异性应激反应有关的常见基因和通路簇。我们的分析揭示了一种时间编排,从伴侣蛋白的上调开始,到随后对运动和趋化途径的抑制,以及酶和生物膜调节剂厌氧池的诱导。对转录数据的系统分析发现,zur调节的基因簇在针对次氯酸钠和过乙酸的应激反应中高度活跃,表明杀菌剂应激反应与锌稳态之间的联系。敲除突变体的敏感性测定进一步验证了我们的发现,并为下游研究所涉及的作用机制提供了明确的目标。