In this paper, we reveal and characterize cross‐feeding behaviour between the common gut commensal Bacteroides cellulosilyticus (Baccell) and certain bifidobacterial strains, including Bifidobacterium breve UCC2003, when grown on a medium containing Larch Wood Arabinogalactan (LW‐AG). We furthermore show that cross‐feeding is dependent on the release of β‐1,3‐galacto‐di/trisaccharides (β‐1,3‐GOS), and identified that the bga gene cluster of B. breve UCC2003 allows β‐1,3‐GOS metabolism. The product of bgaB is presumed to be responsible for the import of β‐1,3‐GOS, while the bgaA gene product, a glycoside hydrolase family 2 member, was shown to hydrolyse both β‐1,3‐galactobiose and β‐1,3‐galactotriose into galactose monomers. This study advances our understanding of strain‐specific syntrophic interactions between two glycan degraders in the human gut in the presence of AG‐type dietary polysaccharides.

中文翻译：

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在源自植物的阿拉伯半乳聚糖上培养时，两个常见肠道菌之间的交叉进食行为的生化分析。

在本文中，我们揭示并表征了当在含有落叶松木阿拉伯半乳聚糖（LW‐AG）的培养基上生长时，常见的肠道共生纤维素分解杆菌（Baccell）与某些双歧杆菌菌株（包括短双歧杆菌UCC2003）之间的交叉喂养行为。我们进一步证明，交叉喂养取决于β-1,3-半乳糖二糖/三糖（β-1,3-GOS）的释放，并确定短双歧杆菌UCC2003的bga基因簇允许β-1 ，3-GOS代谢。假定bgaB的产物负责β-1,3-GOS的进口，而bgaA的产物基因产物是糖苷水解酶家族2成员，已显示可将β-1,3-半乳糖二糖和β-1,3-半乳糖三糖水解为半乳糖单体。这项研究使我们对在存在AG型饮食多糖的情况下人类肠道中的两个聚糖降解物之间的菌株特异性突触相互作用进行了了解。