Bacterial canker is a major disease of Prunus species, such as cherry (Prunus avium). It is caused by Pseudomonas syringae pathovars, including P. syringae pv. syringae (Pss) and P. syringae pv. morsprunorum race 1 (Psm1) and race 2 (Psm2). Concerns over the environmental impact of, and the development of bacterial resistance to, traditional copper controls calls for new approaches to disease management. Bacteriophage‐based biocontrol could provide a sustainable and natural alternative approach to combat bacterial pathogens. Therefore, seventy phages were isolated from soil, leaf and bark of cherry trees in six locations in the south east of England. Subsequently, their host range was assessed against strains of Pss, Psm1 and Psm2. While these phages lysed different Pss, Psm and some other P. syringae pathovar isolates, they did not infect beneficial bacteria such as Pseudomonas fluorescens. A subset of thirteen phages were further characterized by genome sequencing, revealing five distinct clades in which the phages could be clustered. No known toxins or lysogeny‐associated genes could be identified. Using bioassays, selected phages could effectively reduce disease progression in vivo, both individually and in cocktails, reinforcing their potential as biocontrol agents in agriculture.

中文翻译：

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噬菌体生物防治技术可与丁香假单胞菌病原体对抗，从而引起樱桃疾病。

细菌性溃疡病是李属物种的主要疾病，例如樱桃（Prunus avium）。它是由丁香假单胞菌病引起的，包括丁香假单胞菌PV。丁香（Pss）和丁香假单胞菌pv。赛车种族1（Psm1）和种族2（Psm2）。对传统铜质控制措施的环境影响和细菌耐药性的发展的担忧，要求采用新的疾病管理方法。基于噬菌体的生物防治可以提供一种可持续的天然替代方法来对抗细菌病原体。因此，在英格兰东南部的六个地方，从土壤，樱桃树的树皮和树皮中分离出了70个噬菌体。随后，针对Pss，Psm1和Psm2菌株评估了它们的宿主范围。尽管这些噬菌体裂解了不同的Pss，Psm和其他丁香假单胞菌病原分离株，但它们并未感染有益细菌，例如荧光假单胞菌。通过基因组测序进一步表征了十三个噬菌体的一个子集，揭示了五个不同的进化枝，这些噬菌体可以在其中聚集。没有已知的毒素或溶原相关基因。使用生物测定法，选定的噬菌体可以有效地减少体内疾病的进展，无论是单独还是在鸡尾酒中，增强其作为农业生物防治剂的潜力。