Mesenchymal stem cells with differentiation ability to diverse cells play a crucial role in tissue engineering. Tracking the fate of these cells during the regeneration of tissue helps to obtain more information about their function. In this study, histidine conjugated β-cyclodextrin as a cell-penetrating carrier with drug loading ability was attached to QDs nanoparticle (QD-βCD-His) for stem cell labeling. Traceability of QD-βCD-His labeled human adipose stem cells (hASCs) was monitored in 2D cell culture and 3D temperature-sensitive chitosan hydrogel scaffold. Dexamethasone (Dex) as an osteoinductive drug was loaded into QD-βCD-His nano-carrier (QD-βCD-His@Dex) to induce bone differentiation of labeled cells. Overall results indicated that QD-βCD-His@Dex is a promising dual-purpose nano-carrier for stem cell labeling with osteoinductive potential in cell therapy as well as tissue engineering scaffolds.

具有向多种细胞分化能力的间充质干细胞在组织工程中起着至关重要的作用。在组织再生期间跟踪这些细胞的命运有助于获得有关其功能的更多信息。在这项研究中，将组氨酸缀合的β-环糊精作为具有药物负载能力的细胞穿透载体，将其附着到QDs纳米颗粒（QD-βCD-His）上以进行干细胞标记。在2D细胞培养和3D温度敏感的壳聚糖水凝胶支架中监测QD-βCD-His标记的人脂肪干细胞（hASCs）的可追溯性。地塞米松（Dex）作为骨诱导药物被加载到QD-βCD-His纳米载体（QD-βCD-His@ Dex）中，以诱导标记细胞的骨分化。