This study evaluated the sustainability of biological mine drainage (MD) treatment using industrial by-products. Crude glycerol (CG) from biodiesel production and trub—a brewery waste — were used as electron donors for the biological reduction of sulfate. Batch assays were prepared using anaerobic inoculum (from a domestic sewage treatment plant) and mine drainage (from a gold mining company). The MD showed high concentrations of pollutants (in mg.L⁻¹): 3,860.5 sulfate, 1.39 arsenic, 14.77 cadmium, 1.78 lead, 3.85 antimony, and 209.03 zin. Under a chemical oxygen demand/SO₄²⁻ ratio of 3.2, sulfate removal was similar among tested substrates and positive controls of pure glycerol (PG) and ethanol, between 89.5 and 92.2 % of sulfate removed. The removal of Pb using crude glycerol was 88.3 %, while that for the other metals was above 99 %. On the other hand, the removal of all metals was over 99 % using trub. The substrates CG and trub were good carbon sources as compared to the positive controls. It is thus believed that industrial by-products are as effective as positive controls in making the MD treatment more sustainable by eliminating organic substrate acquisition costs and the consumption of products requiring treatment before environment discharge. To obtain 1 g of COD with trub, 538 mL of product is consumed at no cost. To obtain the same COD value with CG, PG and ethanol is necessary to invest US $ 0.0053, 0.1184 and 14.4, respectively.

这项研究评估了使用工业副产品进行生物矿山排水（MD）处理的可持续性。生物柴油生产中的粗甘油（CG）和啤酒废料trub被用作电子还原硫酸盐的电子供体。使用厌氧接种物（来自生活污水处理厂）和矿井排水（来自金矿开采公司）进行批量测定。MD显示出高浓度的污染物（以mg.L ^-1计）：硫酸3,860.5、1.39砷，14.77镉，1.78铅，3.85锑和209.03锌。在化学需氧量/ SO ₄ ^2-下在3.2的比率下，在测试的底物和纯甘油（PG）和乙醇的阳性对照之间，硫酸盐的去除率相似，在89.5-92.2％的硫酸盐去除率之间。使用粗甘油去除的Pb为88.3％，而其他金属的去除率则高于99％。另一方面，使用t钻去除所有金属的比例超过99％。与阳性对照相比，底物CG和trub是良好的碳源。因此，据信，通过消除有机底物的购置成本和环境排放前需要处理的产品的消耗，工业副产品与阳性对照一样有效，可以使MD处理更具可持续性。为了通过搅打获得1 g的COD，无偿消耗了538 mL的产品。要获得与CG，PG和乙醇相同的COD值，需要投资0.0053美元，0。