Scutellum derived calli of recalcitrant indica rice variety ASD-16 are subjected to qualitative and quantitative changes using different callus induction media (CIM). The suitable media for generation of regenerating calli by evaluating the increase in size of these calli as a function of time (Meazure^TM2.0 software), were recorded (till 25-days post-inoculation). After 10-days post-inoculation significant differences which ranged from 5 mm to 6.5 mm and 30% variation in calliQuery size were recorded for different CIM. Improved regeneration achieved by reducing the time on callusing media to 5-days and 10-days. Also, the insights are provided for the role of cationic and anionic strength, phenomics of somatic embryogenesis, and also browning of the calli for recalcitrant indica rice variety ASD-16. The statistical analysis of size of calli with ionic strength of cations K⁺, H⁺, NH₄⁺, Mg²⁺, Ca²⁺ and anions PO₄³⁻, NO₃⁻, Cl⁻ (statistical analysis tool “The Unscramble X”) shows positive correlation. The loss of scutellum derived calli due to browning was reduced by allowing the mature seed used for generation of calli to be attached to the growing calli. The browning of the calli was monitored in different media for the pattern, and statistical evidences are provided for the important role played by ionic ratios of media constituent namely, NH₄⁺/NO₃⁻ and SO₄²⁻/PO₄³⁻ (reported here for the first time). Maximum healthy calli obtained (80%) were on CIM-2 whereas maximal browning (60%) was obtained on CIM-4 after 15-days post-inoculation. Successful regeneration is achieved for recalcitrant indica rice variety ASD-16.

盾片来源的愈伤组织的顽抗籼水稻品种ASD-16经受使用不同的愈伤组织诱导培养基（CIM）的定性和定量的变化。记录了通过评估这些愈伤组织的大小随时间的变化而生成再生愈伤组织的合适培养基（Meazure ^TM 2.0软件）（接种后至25天）。接种后10天后，对于不同的CIM，记录了5mm至6.5mm的显着差异，并且calliQuery大小发生了30％的变化。通过将调用媒体的时间减少到5天和10天来实现更好的再生。此外，还提供了有关阳离子和阴离子强度的作用，体细胞胚发生的表观性以及顽固的愈伤组织褐变的见解。籼水稻品种ASD-16。阳离子K ⁺，H ⁺，NH ₄ ⁺，Mg 2 ⁺，Ca ²⁺和阴离子PO ₄ ³⁻，NO ₃ ⁻，Cl ^−的离子强度的愈伤组织大小的统计分析（统计分析工具“ The Unscramble X ”）显示正相关。通过使用于产生愈伤组织的成熟种子附着在生长的愈伤组织上，减少了由于褐变引起的盾片来源愈伤组织的损失。在不同的培养基中监测了愈伤组织的褐化情况，并提供了统计依据，证明了培养基成分即NH的离子比率所起的重要作用。₄ ⁺ / NO ₃ ^-和SO ₄ ^2- / PO ₄ ^3-（此处报道首次）。接种后15天，在CIM-2上获得的最大健康愈伤组织（80％），而在CIM-4上获得的最大褐变（60％）。顽calc稻品种ASD-16已成功再生。