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Production of protein hydrolysate from Protaetia brevitarsis seulensis (Kolbe) larvae by enzyme treatment under high pressure.
Food Science and Biotechnology ( IF 2.9 ) Pub Date : 2020-05-08 , DOI: 10.1007/s10068-020-00766-y
Tae-Eun Kim 1 , Chong-Tai Kim 2 , Hyeon-Jae Sim 3 , Hoon-Yeon Lee 3 , Seong-Oh Kim 3 , Dong-Keun Kim 3 , Mi-Na Jo 3 , So-Jin Lee 3 , Yong-Deok Jeon 4, 5 , Young-Jae Song 4 , Jong-Sik Jin 4 , Yong-Jin Cho 1
Affiliation  

To improve the industrial use of health-functional materials based on edible insects, the objective of this study was to establish optimal conditions for improving the quality of Protaetia brevitarsis seulensis larval (PBSL) hydrolysates. PBSL was extracted using four methodologies: atmospheric pressure 50 °C-water extraction, atmospheric pressure 95 °C-water extraction, atmospheric pressure 50 °C-water enzymatic hydrolysis, and enzyme treatment under high pressure (HPE). The quality characteristics of soluble solid content, extraction yield, total protein content, protein yield, protein content with low molecular weight (LMW) (< 1kD), and the amino acid composition of hydrolysates were compared based on the different methods. All of the quality characteristics were found to be higher for HPE extracts than for the other extracts. Under optimized HPE conditions, extraction yield, protein yield, protein content with LMW, amino acid content and the content of essential amino acids increased by 3.4, 4.4 1.4 1.5, and 1.3 times respectively, compared to the other methods.



中文翻译:

通过高压酶处理,由短小丙酸杆菌(Protaetiabrevitarsisseulensis)(Kolbe)幼虫生产蛋白质水解产物。

为了改善以食用昆虫为基础的保健功能材料的工业用途,本研究的目的是为提高短杆菌Protaetia brevitarsis seulensis)的质量建立最佳条件。幼虫(PBSL)水解产物。使用四种方法提取PBSL:大气压50°C-水提取,大气压95°C-水提取,大气压50°C-水酶水解和高压酶处理(HPE)。根据不同的方法,比较了可溶性固形物含量,提取率,总蛋白含量,蛋白产量,低分子量(LMW)(<1kD)的蛋白含量和水解产物的氨基酸组成的质量特征。发现HPE提取物的所有质量特性均高于其他提取物。在优化的HPE条件下,提取产量,蛋白质产量,含LMW的蛋白质含量,氨基酸含量和必需氨基酸含量分别增加了3.4倍,4.4倍,1.5倍和1.3倍,

更新日期:2020-05-08
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