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Comparison of mRNA Expression of P2X Receptor Subtypes in Different Arterial Tissues of Rats.
Biochemical Genetics ( IF 2.4 ) Pub Date : 2020-05-08 , DOI: 10.1007/s10528-020-09968-9 Ling-Zhi Li 1 , Li-Hui Yue 2 , Zhi-Min Zhang 1 , Jing Zhao 3 , Lei-Ming Ren 3 , Hong-Jie Wang 1 , Lu Li 1
Biochemical Genetics ( IF 2.4 ) Pub Date : 2020-05-08 , DOI: 10.1007/s10528-020-09968-9 Ling-Zhi Li 1 , Li-Hui Yue 2 , Zhi-Min Zhang 1 , Jing Zhao 3 , Lei-Ming Ren 3 , Hong-Jie Wang 1 , Lu Li 1
Affiliation
This study aims to compare the expression of P2X receptor subtype mRNA in different arterial tissues of rats. After the rats were sacrificed, the internal carotid, pulmonary, thoracic aorta, mesenteric and caudal arteries were dissected out. Then, the P2X receptor mRNA expression in different blood vessels was detected by reverse transcription-polymerase chain reaction (RT-PCR) and real-time quantitative polymerase chain reaction. The P2X1, P2X4 and P2X7 receptor mRNA amplification products revealed specific bands of the same size as the amplified target fragment in their respective lanes, while the P2X2, P2X3, P2X5 and P2X6 receptor mRNA amplification products did not reveal significant specific bands in their respective lanes by RT-PCR. Based on the P2X1 receptor mRNA expression of the mesenteric artery, there were no significant differences in the internal carotid, pulmonary and thoracic aorta (0.64 ± 0.07, 0.17 ± 0.11 and 1.49 ± 0.65, respectively). However, the P2X1 receptor mRNA expression level in the caudal artery significantly increased (11.06 ± 1.99, P < 0.01). Furthermore, there was no difference in P2X4 receptor mRNA expression among these five blood vessels (P > 0.05). The P2X7 receptor mRNA expression level was significantly different: pulmonary artery < tail artery = thoracic aorta < internal carotid artery < mesenteric artery. The relative P2X1 receptor mRNA expression in the caudal artery was observed to be elevated when compared to that of the internal carotid, pulmonary and thoracic aorta as well as the mesenteric arteries. The P2X7 receptor mRNA expression level is pulmonary artery < caudal artery = thoracic aorta < internal carotid artery < mesenteric artery. P2X4 receptor mRNA expression was not significantly different among these five blood vessels.
中文翻译:
大鼠不同动脉组织中P2X受体亚型mRNA表达的比较。
本研究旨在比较P2X受体亚型mRNA在大鼠不同动脉组织中的表达。处死大鼠后,解剖出颈内动脉,肺动脉,胸主动脉,肠系膜动脉和尾动脉。然后,通过逆转录聚合酶链反应(RT-PCR)和实时定量聚合酶链反应检测不同血管中P2X受体的mRNA表达。P2X1,P2X4和P2X7受体mRNA扩增产物在其各自泳道中显示出与扩增的目标片段大小相同的特定条带,而P2X2,P2X3,P2X5和P2X6受体mRNA扩增产物在其各自泳道中未显示出明显的特异性条带。通过RT-PCR。根据肠系膜动脉的P2X1受体mRNA表达,颈内,肺和胸主动脉无显着差异(分别为0.64±0.07、0.17±0.11和1.49±0.65)。然而,尾动脉中的P2X1受体mRNA表达水平显着增加(11.06±1.99,P <0.01)。此外,这五个血管之间的P2X4受体mRNA表达没有差异(P> 0.05)。P2X7受体的mRNA表达水平差异显着:肺动脉<尾动脉=胸主动脉<颈内动脉<肠系膜动脉。与颈内动脉,肺和胸主动脉以及肠系膜动脉相比,尾动脉中相对P2X1受体mRNA的表达升高。P2X7受体mRNA表达水平为肺动脉< 尾动脉=胸主动脉<颈内动脉<肠系膜动脉。在这五个血管之间,P2X4受体mRNA表达没有显着差异。
更新日期:2020-05-08
中文翻译:
大鼠不同动脉组织中P2X受体亚型mRNA表达的比较。
本研究旨在比较P2X受体亚型mRNA在大鼠不同动脉组织中的表达。处死大鼠后,解剖出颈内动脉,肺动脉,胸主动脉,肠系膜动脉和尾动脉。然后,通过逆转录聚合酶链反应(RT-PCR)和实时定量聚合酶链反应检测不同血管中P2X受体的mRNA表达。P2X1,P2X4和P2X7受体mRNA扩增产物在其各自泳道中显示出与扩增的目标片段大小相同的特定条带,而P2X2,P2X3,P2X5和P2X6受体mRNA扩增产物在其各自泳道中未显示出明显的特异性条带。通过RT-PCR。根据肠系膜动脉的P2X1受体mRNA表达,颈内,肺和胸主动脉无显着差异(分别为0.64±0.07、0.17±0.11和1.49±0.65)。然而,尾动脉中的P2X1受体mRNA表达水平显着增加(11.06±1.99,P <0.01)。此外,这五个血管之间的P2X4受体mRNA表达没有差异(P> 0.05)。P2X7受体的mRNA表达水平差异显着:肺动脉<尾动脉=胸主动脉<颈内动脉<肠系膜动脉。与颈内动脉,肺和胸主动脉以及肠系膜动脉相比,尾动脉中相对P2X1受体mRNA的表达升高。P2X7受体mRNA表达水平为肺动脉< 尾动脉=胸主动脉<颈内动脉<肠系膜动脉。在这五个血管之间,P2X4受体mRNA表达没有显着差异。