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Treatment with disinfectants may induce an increase in viable but non culturable populations of Listeria monocytogenes in biofilms formed in smoked salmon processing environments.
Food Microbiology ( IF 5.3 ) Pub Date : 2020-05-08 , DOI: 10.1016/j.fm.2020.103548
Thomas Brauge 1 , Christine Faille 2 , Guylaine Leleu 1 , Catherine Denis 3 , Aurélie Hanin 3 , Graziella Midelet 1
Affiliation  

The objectives of this study were 1) to evaluate the impact of two industrial disinfectants on the viability of Listeria monocytogenes populations in biofilm and 2) to investigate the viability state of L. monocytogenes cells present on contact surfaces in the smoked salmon processing environment. In the first step, we cultured mono species and mixed species biofilms containing L. monocytogenes on stainless steel or polyvinyl chloride (PVC) at 8 °C for 48h. The biofilms were then exposed to quaternary ammonium- and hydrogen peroxide-based disinfectants. Residual total populations of L. monocytogenes were measured by qPCR, and viable culturable (VC) cell populations were quantified using standard microbiological culture-based techniques and by a quantitative PCR (qPCR) assay coupled with a propidium monoazide treatment. Decreases in VC populations and the appearance of viable but non culturable (VBNC) populations were observed in response to treatment with the disinfectants. An 8 month sampling campaign in 4 smoked salmon processing plants was also carried out to detect L. monocytogenes in environmental samples. VBNC cells were detected mainly after the cleaning and disinfection operations. This study showed that industrial disinfectants did not inactivate all L. monocytogenes cells on inert surfaces. The presence of VBNC populations of L. monocytogenes in the smoked salmon processing environment is a public health concern.



中文翻译:

用消毒剂处理可能会导致烟熏鲑鱼加工环境中形成的生物膜中单核细胞增生李斯特菌的存活但不可培养的种群增加。

这项研究的目的是1)评估两种工业消毒剂对生物膜中单核细胞增生李斯特菌种群生存力的影响,以及2)调查烟熏三文鱼加工环境中接触表面上存在的单核细胞增生李斯特菌细胞的生存状态。第一步,我们在8°C的不锈钢或聚氯乙烯(PVC)上培养了包含单核细胞增生李斯特菌的单物种和混合物种生物膜48小时。然后将生物膜暴露于季铵盐和过氧化氢基消毒剂。单核细胞增生李斯特氏菌的残留总数通过qPCR测量细胞,并使用基于标准微生物培养的技术和结合单叠氮化丙锭处理的定量PCR(qPCR)测定法对可培养的(VC)细胞群体进行定量。响应消毒剂的处理,观察到VC种群减少,并且出现了可存活但不可培养的(VBNC)种群。在四个熏制鲑鱼加工厂进行了为期8个月的采样活动,以检测环境样品中的单核细胞增生李斯特菌。主要在清洁和消毒操作后检测到VBNC细胞。这项研究表明,工业消毒剂不会使惰性表面上的所有单核细胞增生李斯特菌细胞失活。单核细胞增生李斯特菌的VBNC种群的存在 在烟熏三文鱼加工环境中,这是公共卫生问题。

更新日期:2020-06-28
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