The neurotropic behavior of Chandipura virus (CHPV) is partly understood in experimental animals. Under in vitro conditions, neuronal cells could be a useful tool to study the CHPV interaction with neuronal proteins. The information gathered from such studies will help to design the new therapeutics for CHPV infection. This study identified the surface vimentin protein involved in adsorption of CHPV on Neuro-2a cell line (mouse neuroblastoma cells). The decrease in CHPV infectivity to Neuro-2a cells was observed in the presence of recombinant vimentin or anti-vimentin antibody. Vimentin mRNA expression remains unaltered in CHPV infected Neuro-2a cells. Furthermore, in silico analysis predicted the residues in vimentin and CHPV glycoprotein (G); probably involved in cell-virus interactions. Overall, we conclude that surface vimentin in Neuro-2a cells interact with CHPV and facilitate the binding of CHPV to the cells; it could be acting as a co-receptor for the CHPV. Further investigation is necessary to confirm the exact role of vimentin in CHPV infection in neuronal cells.

中文翻译：

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细胞表面波形蛋白在 Neuro-2a 细胞中金迪普拉病毒复制中的作用。

钱迪普拉病毒 (CHPV) 的嗜神经行为在实验动物中得到部分了解。在体外条件下，神经元细胞可能是研究 CHPV 与神经元蛋白相互作用的有用工具。从这些研究中收集的信息将有助于设计 CHPV 感染的新疗法。该研究鉴定了参与 CHPV 在 Neuro-2a 细胞系（小鼠神经母细胞瘤细胞）上吸附的表面波形蛋白。在重组波形蛋白或抗波形蛋白抗体存在的情况下，观察到 CHPV 对 Neuro-2a 细胞的感染性降低。波形蛋白 mRNA 表达在 CHPV 感染的 Neuro-2a 细胞中保持不变。此外，计算机分析预测波形蛋白和 CHPV 糖蛋白 (G) 中的残留；可能与细胞-病毒相互作用有关。总体，我们得出结论，Neuro-2a 细胞中的表面波形蛋白与 CHPV 相互作用并促进 CHPV 与细胞的结合；它可以作为 CHPV 的共同受体。需要进一步研究以确认波形蛋白在神经元细胞中 CHPV 感染中的确切作用。