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Genomic and biological features of a novel orbivirus isolated from mosquitoes, in China.
Virus Research ( IF 5 ) Pub Date : 2020-05-08 , DOI: 10.1016/j.virusres.2020.197990 De Wu 1 , Qiqi Tan 1 , Huan Zhang 1 , Ping Huang 2 , Huiqiong Zhou 1 , Xin Zhang 1 , Jiufeng Sun 3 , Liming Huang 4 , Guodong Liang 5
Virus Research ( IF 5 ) Pub Date : 2020-05-08 , DOI: 10.1016/j.virusres.2020.197990 De Wu 1 , Qiqi Tan 1 , Huan Zhang 1 , Ping Huang 2 , Huiqiong Zhou 1 , Xin Zhang 1 , Jiufeng Sun 3 , Liming Huang 4 , Guodong Liang 5
Affiliation
A novel orbivirus had been identified as a member of the Orbivirus genus, which was isolated from pooled Culex fatigans mosquitoes in Guangdong of China, named as the Fengkai virus (FKOV). The cytopathic effects (CPEs) on both Aedes albopictus cells (C6/36) and mammalian cell lines (Vero and BHK-21) emerged in the cell cultures inoculated above virus in. Experimental confirmation as the Orbivirus genus was conducted by the Real-time PCR and based on Ion Torrent Next-Generation in sequencing. The Identities of VP1, VP2 and VP3 in amino acid sequences between the Tibet orbivirus (TIBOV) and this strain were 98.6%, 42.9%, and 99.9%, respectively, which indicated that this strain shares the same genus (VP1, Pol) and species (VP3, T2) with TIBOV but was greatly different in VP2 and VP5 (10.3%) of TIBOV. The VP2 and VP5 diversities of both TIBOV and FKOV strains suggested both serotypes are distinct with each other. As natural evolution and circulation, this strain might expand its host ranges and infect human beings as a potential and severe pathogen.
更新日期：2020-05-08
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