This study analyzed the architecture of Platelet-Rich Fibrin (PRF) clots and assessed their elemental composition in order to provide new insight into this biomaterial. Five surplus PRF clots (2,700 RPM, 12 min.) donated by patients (63.6 ± 12.3 years old) were prepared for use in dental clinical procedures. The internal three-dimensional morphology of the red zones and the thirds of the yellow zones of the clots were analyzed by Variable Pressure Scanning Electron Microscope (VPSEM) after sample preparation by two methods: 1. Fixation (2.5% gluataraldehyde); and 2. Fixation with subsequent partial removal of extracellular elements (8 N, HCl). Semi-quantitative elemental analysis was performed by energy-dispersive X-ray spectrometry (EDX). VPSEM analysis showed erythrocytes in both the red zone and the yellow zone, which consisted mainly of fibrin. Removal of extracellular elements enriched the morphology of both zones; the organization of the fibrin was observed to differ in the thirds of the yellow zone, with increasing density and organization to distal. The elements that compose organic substances (C-Carbon, N-Nitrogen, O-Oxygen, Na-Sodium and P-Phosphorus) and halogens (Cl-Chloride and S-Sulfur) were detected; the highest concentrations were of C, followed by O (p < 0.05), in the proximal region of the fibrin. The results of the present study suggest organization of fibrin in the PRF clot, and also reveal the distribution of the elements present in the different regions of the clot. Improved understanding of these characteristics may favor the use of this biomaterial by increasing its efficiency and functionality.

这项研究分析了富含血小板的纤维蛋白（PRF）凝块的结构，并评估了它们的元素组成，以提供对该生物材料的新见解。准备了由患者（63.6±12.3岁）捐赠的五个多余的PRF血凝块（2,700 RPM，12分钟），用于牙科临床程序。样品制备后，采用两种方法通过可变压力扫描电子显微镜（VPSEM）分析血凝块红色区域和黄色区域的三分之一的内部三维形态：1.固定（2.5％戊二醛）；2.固定，随后部分去除细胞外元素（8 N，HCl）。半定量元素分析通过能量色散X射线光谱仪（EDX）进行。VPSEM分析显示红色区域和黄色区域中的红细胞，主要由纤维蛋白组成。去除细胞外元素丰富了两个区域的形态。观察到纤维蛋白的组织在黄色区域的三分之一处有所不同，随着向远端的密度和组织的增加。检测到构成有机物质（碳，氮，氧，钠和磷）和卤素（氯和硫）的元素；在血纤蛋白的近端区域，最高浓度的是C，其次是O（p <0.05）。本研究的结果表明血纤蛋白在PRF血凝块中的组织，并且还揭示了血凝块不同区域中存在的元素的分布。更好地理解这些特性可能会通过提高其效率和功能性来促进这种生物材料的使用。