Greater than optimal diluting of semen for producing a large number of doses containing relatively small numbers of sperm can lead to compromised quality of sperm, post-thawing. In the present study the French mini-straw plug position was modified and the effect of re-positioning was evaluated on the quality of sperm after thawing subsequent to cryopreservation of small doses of sperm. Four types of mini-straws were used based on the position of cotton plug including no plug displacement (Type 1; Manufacturers location for plug-placement in 0.25 mL French mini-straws), and Type II, III, and IV with re-positioning the cotton plug being 2.5, 5, and 7.5 cm, respectively, further from the manufacturer’s placement location. Each ejaculate was proportioned into four Aliquots (I, II, III, and IV) and diluted to 80, 60, 40, and 20, million sperm/mL, respectively. Aliquot I was placed in all types of straws, while Aliquots II, III, IV were placed only in Type I straws. Semen straws were equilibrated, cryopreserved and sperm kinetic and functional variables were evaluated post-thawing. The results indicate that in Aliquots III and IV there were lesser (P < 0.05) values for sperm kinetic and function variables compared with sperm from Aliquot I (i.e., unmodified mini-straw). In conclusion, cryopreservation of small doses of sperm in modified French mini-straws resulted in acceptable values for kinetic and function variables, post-thawing.

为了产生大量含有相对较少数量精子的剂量，精液的最佳稀释程度超过最佳值，可能会导致解冻后精子质量下降。在本研究中，修改了法国迷你吸管的位置，并评估了冷冻后小剂量精子解冻后重新定位对精子质量的影响。根据棉塞的位置使用了四种类型的微型吸管，包括无塞子位移（类型1；制造商将塞子放置在0.25 mL法国微型吸管中的位置），以及II，III和IV型（可重新定位）棉塞距制造商的放置位置分别为2.5厘米，5厘米和7.5厘米。将每个射精按比例分成四个等分试样（I，II，III和IV），并稀释至80、60、40和20百万精子/ mL，分别。等分试样I放置在所有类型的吸管中，而等分试样II，III，IV仅放置在I型吸管中。平衡精液秸秆，冷冻保存，解冻后评估精子动力学和功能变量。结果表明，等分试样III和IV中的含量较少（与来自等分试样I（即未修饰的小吸管）的精子相比，精子动力学和功能变量的P <0.05）值。总之，在改良的法国迷你吸管中冷冻保存小剂量的精子可得到解冻后动力学和功能变量的可接受值。